Effects of TNF-α on lrg expression in human cell lines
AIM:To examine the effect of tumor necrosis factor α (TNF-α) on lipopolysaccharide response gene (lrg) expression in human cell lines HEK293 and U937. METHODS: Human embryonic cell line HEK293 and leukemia cell line U937 were cultured in RPMI 1640 with 100 mL/L fetal serum under 50 mL/L CO2 condition. The cells were treated with TNF-α at the concentration of 1 × 106 U/L or placebo for 2 h. Western Blot and RT-PCR were performed to detect the lrg expression. β-actin was applied as an internal control. RESULTS: The results of Western Blot indicated that the lrg expression in both HEK293 and U937 cells at protein levels were up-regulated significantly after stimulation by TNF-α for 2 h. The results of RT-PCR indicated that the lrg mRNA levels in both HEK293 and U937 cells increased after stimulation by TNF-α for 2 h. CONCLUSION: The lrg expression in human HEK293 and U937 cells is both up-graded remarkably by the stimulation of TNF-α, which suggests that lrg probably plays roles in the inflammation induced by TNF-α.
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