Ultrasound-targeted microbubble destruction combined with granulocyte colony stimulating factor facilitates angiogenesis of infarcted myocardium in rats
AIM: To explore whether ultrasound-targeted micro-bubble destruction ( US/MB) combined with granulocyte colony-stimulating factor ( G-CSF) can facilitate secretion of vascular endothelial growth factor ( VEGF) and angiogenesis of the infarcted myocardium in rats. METHODS: Forty myocardial infarction Wistar rats models were randomly divided into 4 groups: US/MB + G-CSF treatment group (A); US/MB alone treatment group ( B); G-CSF alone treatment group (C); and surgery alone (SA) group (D). Two weeks later, the rats were killed and the myocardia were harvested. The CD34 expression was detected by immunohistochemistry, and microvessel density ( MVD) was counted in high-power field ( HPF) under microscope. The VEGF expression in myocardium was detected by ELISA. RESULTS: There was significant CD34 expression in the myocardium of group A, and the MVD was higher in group A [ (231 ±24)/HPF] than in group B[( 148 ±19)/HPF], C[ (100 ±12)/HPF] and [(86 ±8)/HPF](P<0.05). The content of VEGF in the myocardium of group A[ (3. 14 ±0. 21) ng/g] was higher than those in group B[ (2.56 ±0.17)ng/g], C[ (1.91 ±0. 14)ng/g ], and D[(0.97±0.11)ng/g](P<0.05). CONCLUSION: US/MB can facilitate the endogenous secretion of VEGF and accelerate the vascularization to the infarcted myocardium in rats. Pretreatment with G-CSF can augment the angiogenic effect of US/MB.
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万方数据
维普
