Isolation, culture of Nestin positive cells and Nestin expression in human neuroepithelial tumors
AIM: To investigate the expression of Nestin in human neuroepithelial tumors and to evaluate the biological characteristics of Nestin positive tumor stem cells in vitro and the significance. METHODS : Nestin expression was detected in 33 cases of neuroepithelial tumors using the immunocytochemical staining. The Nestin positive and negative cells were isolated by magnetic activated cell sorting ( MACS). Nestin positive cells were culutered, identified, and induced to differentiate. The expression of Nestin protein was detected in Nestin + cells by RT-PCR. RESULTS: All the vascular endothelial cells of tumors were positive for Nestin. The percentage of Nestin positive cells was 9.5% - 96. 2% in the neuroepithelial tumors. The positive expression rate and staining intensity of Nestin + cells were positively correlated with the tumor pathological grades. Nestin protein activity in Nestin + tumor stem cells was 18.5 times more than that of Nestin cells, and expression intensity was related to cell origin. Nestin positive cells proliferated into form typical tumor neurospheres in serum-free medium and further differentiated into neuron-like, glia-like and oligodendroglia-like mature cells. CONCLUSION:The detection of Nestin expression contributes to correctly evaluate the malignant degree and biological behaviors and is an important clue to ascertain the pathological grades and prognosis of neuroepithelial tumors.
neurvow system neoplasmsNestinpathological gradebrain tumor stem cellsprognosis
NETL
NSTL
万方数据
维普
