Effects of pioglitazone on proliferation, apoptosis and invasiveness of QBC939 in vitro
AIM: To explore the effects of peroxisome prolifera-tor-activated receptor-γ (PPAR-γ) ligand pioglitazone (PGZ) on the proliferation, apoptosis and invasiveness of human bile duct carcinoma cell line QBC939 in vitro. METHODS: QBC939 cells were cultured in vitro. The effects of PGZ on the growth of QBC939 cells were examined by TUNEL and flow cytometry. The influences of PGZ on the invasiveness and mobility of QBC939 cells were detected by invasion assay and crossing-river test respectively. RESULTS: According to the flow cytometry, the cell proliferation of QBC939 was significantly inhibited by PGZ, while the ratio of cells in G2/M phase was obviously increased. TUNEL displayed the apoptosome in the apoptotic QBC 939 cells. And also, PGZ induced the apoptosis of the tumor cells. Matrigel invasion assay and crossing-river test suggested that, with the increase of PGZ concentration, cells breaking through the Matrigel were reduced significantly and the time crossing river was prolonged ( P < 0. 01 ), with a relationship of dosage-effect. CONCLUSION: PPAR-γ ligand PGZ can inhibit QBC939 cells' proliferation, induce their apoptosis, and reduce their invasiveness and mobility in vitro. So, we infer that PGZ may be a new point to treat bile duct carcinoma.
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万方数据
维普
