Effects of angelica polysaccharides on expression of adhesion molecules and adhesive function of murine bone marrow stromal cells
AIM: To explore the effects of angelica polysaccharides ( APS) on the cell growth, the expression of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule -1 ( VCAM-1) in marine bone marrow stromal cells ( BMSCs) and the adhesive rate of murine bone marrow mononuclear cells to BMSCs. METHODS:The effects of APS on the digestion time of the murine BMSCs were observed with inverted microscope. The proliferation of BMSCs and the binding capacity of murine bone marrow mononuclear cells to BMSCs were evaluated by MTT assay. The expressions of ICAM-1 and VCAM-1 in murine BMSCs were analyzed by flow cytometry. RESULTS: With 100, 200 mg/L of APS, the digestion time of murine BMSCs obviously decreased compared with control group. The cultured cells proliferated well in DMEM/F12 containing 50 and 100 mg/L APS. 200 and 300 mg/L of APS decreased the expression of ICAM-1. 50,100,200 and 300 mg/L of APS decreased the expression of VCAM-1. The expressions of ICAM-1 and VCAM-1 were also decreased by intra-peritoneal injection of APS. With 100, 200 and 300 mg/L of APS, the adhesive capacity of BMSCs to murine bone marrow mononuclear cells was inferior to control group. CONCLUSION: APS enhances the proliferation of BMSCs and decreases the expression of ICAM-1 and VCAM-1 in BMSCs, so as to shorten the digestion time of murine BMSCs, and decrease the adhesive rate of murine bone marrow mononuclear cells to BMSCs.
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