Effects of Dibutyl phthalate on procreant ability and its genotoxity in male Drosophila melanogaster
AIM: To study the effects of dibutyl phthalate (DBP) on the procreant ability and its genotoxity in male Drosophila melanogaster. METHODS :①The non-copulated male Drosophila melanogasters were divided randomly into 5 groups and were continuously fed with medium containing 0,1,4,16,64 mg/g DBP for 10 days. Then the treated male flies were brought to the copulation trial and the reproduction trial. ②The male Drosophila melanogasters were divided randomly into 4 groups and were continuously fed with solution containing 0,50,200,800 mg/L DBP for 3 days. The mutation-inducing effects of DBP was observed using a Sex-Linked Recessive Lethal Test(SLRL test) in Drosophila melanogaster. RESULTS:The mating rate in 1 hour and the average reproduction of male flies in DBP groups were gradually decreased with the rising of DBP concentration. The mating rate in 1 hour (40%) and the average reproduction (486 ±265) of male flies in 64 mg/g DBP group were lower significantly than those in control group respectively [ 85% , ( 861 ± 312), P < 0.05]. During the first 10 days of the procreation period, the yield rate of male flies in DBP groups were obviously higher as compared with control group ( P < 0. 01 ). After continuously procreating for 10 days, the yield rate of male flies merely in higher concentration of DBP groups were significantly higher than that of control group( P < 0. 05). The mutation rates (0. 990% ) of DBP at 50 mg/L concentration in third brood were significantly higher as compared with control group (0. 106% , P < 0. 01). CONCLUSION: DBP might reduce the procreant ability and bring out genotoxity effect on male Drosophila melanogaster.
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万方数据
维普
