Effects of two different antigen-loading methods on activity of dendritic cell vaccine for LNCaP cell line inhibition in vitro
AIM: To observe the changes in the activity of dendritic cells(DCs) vaccine after prostate specific antigen(PSA) gene transfection mediated by recombinant adeno-associated virus type2(rAAV) and tumor cell lysate. METHODS: Immature DCs were isolated from peripheral blood monocytes of healthy volunteers positive with HLA-A2, and were infected with the recombinant adeno-associated virus carrying PSA gene or loaded with tumor cell lysate. Then cytotoxic T cells (CTLs) were induced by these 2 kinds of DCs. The activity of DCs and CTLs were checked, meanwhile the specific killing activity of 2 kinds of CTU to LNCaP cells and the former to LNCaP and DU145 cells were assessed by MTT assay. RESULTS: Mature DCs were obtained in both groups, but DCs in the virus transfection group were more mature and induced more CTLs. The killing effect of CTLs resulting from rAAV transfection of the DCs was more significant than that induced by tumor cell lysate loading, and CTLs transfected by rAAV/PSA specifically recognized LNCaP cells and killed them. CONCLUSION: Both methods of antigen loading can induce mature DCs from peripheral blood monocytes, but rAAV infection of the DCs is more effective than tumor cells lysate loading. DCs transfected with rAAV may have the potency to serve as an adjuvant immunotherapy for patients with prostate cancer.
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万方数据
维普
