Differentiation of adipose-derived stem cells into nerve stem cells across embryonic layer
AIM: To explore the feasibility of differentiation of adipose-derived stem cells (ADSCs) into neural stem cells (NSCs), so as to search the perfect source of seed cells to repair the tissue injury of the central nervous system. METHODS: ADSCs, derived from the adipose of rat's groin, were induced in NSC-culture medium, and then the differentiated cells were identified with immunofluorescence. Proliferation of NSCs was evaluated by formation of cell clones. Antibodies against Nestin, NSE and CFAP were used for identifying NSCs, neurons and glial cells, respectively. RESULTS: Among the rat ADSCs, there were many with rough and large cytoplasmic granules, which proliferated rapidly into islets-shaped cellular spheres with positive expression of Nestin, a specific antigen for NSCs. After separating the cellular spheres into single cells and then plating them, we found the islets-shaped cellular spheres formed again. Plated in the DMEM/F12 culture medium which contained 150 mL/L of serum, some of them formed small buds, which then developed further into long projects connecting each other. Most of the cells with long projects showed positive expression of NSE or GFAP. CONCLUSION: It is possible that ADSCs could differentiate into NSCs under certain experimental conditions. ADSCs might be used as the seed-cells of the neural stem cells for repair of the tissue injury of the central nervous system.
NETL
NSTL
万方数据
维普
