Viability of rabbit thyroid cartilage preserved in vitro
AIM: To study the possibility of vital rabbit thyroid cartilage preservation in vitro using different methods. METHODS:The normal rabbit thyroid cartilages (n = 15) were obtained and the size of cartilage fragments was about 5 mm × 2 mm × 1 mm. The fragments were divided into 3 groups at random and then were separately preserved at 4℃ , - 80℃ and in RPMI-1640 medium at 37℃. The viability of the cartilage grafts was compared after 1,7, 14, 21 and 30 d, using histological method (HE, AB/PAS, Masson stainings) and trypan blue dye exclusion test during the whole 30 d storage duration. RESULTS: The viability of catilage samples kept in RPMI-1640 medium was reserved best (about 85%). Cartilages kept at 4℃ for 30 d showed a quick decrease of viability, and the ones preserved at -80℃ for 7 d lost their viability. CONCLUSION: Compared with low-temperature preservation, tissue culture method can successfully keep the viability of cartilages for a long time. It may offer a new vital cartilage preservation method for clinical application.
NETL
NSTL
万方数据
维普
