摘要
目的:研究如何在体外长期保存兔甲状软骨块的活性.方法:取兔甲状软骨15块,软骨大小约5 mill×2 mill×1mm,将所取软骨块分别置于4℃,-80℃冷冻及RPMI-1640液,于37℃培养保存,于保存24 h,7,14,21,30 d时取样品,分别用HE,AB/PAS及Masson三色染色和台盼蓝排斥试验检测软骨活性.结果:用RPMI-1640培养液保存的兔甲状软骨,在整个保存期中活性保持较好,软骨细胞活性率保持在85%左右;-80℃冷冻保存7d时软骨基本失去活性,4℃保存软骨在保存30d时软骨活性明显降低.结论:与低温保存方法相比,用组织培养法能较好地保存兔甲状软骨块活性.
Abstract
AIM: To study the possibility of vital rabbit thyroid cartilage preservation in vitro using different methods. METHODS:The normal rabbit thyroid cartilages (n = 15) were obtained and the size of cartilage fragments was about 5 mm × 2 mm × 1 mm. The fragments were divided into 3 groups at random and then were separately preserved at 4℃ , - 80℃ and in RPMI-1640 medium at 37℃. The viability of the cartilage grafts was compared after 1,7, 14, 21 and 30 d, using histological method (HE, AB/PAS, Masson stainings) and trypan blue dye exclusion test during the whole 30 d storage duration. RESULTS: The viability of catilage samples kept in RPMI-1640 medium was reserved best (about 85%). Cartilages kept at 4℃ for 30 d showed a quick decrease of viability, and the ones preserved at -80℃ for 7 d lost their viability. CONCLUSION: Compared with low-temperature preservation, tissue culture method can successfully keep the viability of cartilages for a long time. It may offer a new vital cartilage preservation method for clinical application.
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维普
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