Biological effects of curcumin on fibroblasts derived from hypertrophic scars in vitro
AIM: To evaluate the inhibitory effects of the curcumin against hypertrophic scar in vitro. METHODS: Hypertrophic scar fibroblasts ( HSFB), as target cells, were exposed to different concentrations of curcumin respectively. The proliferation of human HSFB was evaluated by methyl thiazolyl tetrazolium (MTT) colorimetric assay. The morphological characters of hypertrophic scar fibroblasts were analyzed by electron microscopy. The apoptosis induced by curcumin were studied by combination of flow cytometry (FCM) and Annexin V/Propidium Iodide double staining. The expressions of procollagen Ⅰ,procollagen Ⅲ and B-cell lymphoma/leukemia-2 (Bcl-2) in HSFB were detected by real-time PCR. RESULTS: Curcumin had a significant inhibitory effect on the proliferation of human HSFB. The number of apoptotic HSFB increased with elevation of the concentration of the curcumin, and these cells appeared typical ultrastructural features. The results of FCM indicated that the apoptosis of the HSFB in vitro was accelerated significantly by curcumin; Curcumin obviously inhibited the expression of the amount of type Ⅰ and Ⅲ procollagen, Bcl-2 mRNA of fibroblasts derived from hypertrophic scar. CONCLUSION: The curcumin inhibits hypertrophic scar in vitro to some extent.
NETL
NSTL
万方数据
维普
