姜黄素对体外培养的增生性瘢痕成纤维细胞的生物学作用
Biological effects of curcumin on fibroblasts derived from hypertrophic scars in vitro
胡晓龙 1胡大海 1计鹏 1蔡维霞 1白晓智 1杨崇志 1韩勇彬1
作者信息
- 1. 第四军医大学西京医院烧伤与皮肤外科,陕西,西安,710033
- 折叠
摘要
目的:观察姜黄素体外抗增生性瘢痕的作用.方法:以增生性瘢痕成纤维细胞为靶细胞,分别用不同浓度的姜黄素培养液培养,MTT法测定细胞的增殖情况,透射电镜进行形态学检测,流式细胞仪技术结合PI及Annexin V双标记染色检测姜黄素的促凋亡作用,实时荧光定量聚合酶链反应检测Ⅰ,Ⅲ型前胶原及Bcl-2 mRNA的表达情况.结果:姜黄素对增生性瘢痕成纤维细胞的增殖有抑制作用;透射电镜观察发现,处理组凋亡细胞增多,凋亡细胞表现出典型的超微结构特征;流式细胞仪检测发现,随着药物浓度的增加,增生性瘢痕成纤维细胞的凋亡率也在逐渐升高;实时荧光定量聚合酶链反应检测结果显示,姜黄素处理后Ⅰ、Ⅲ型前胶原及Bcl-2 mRNA的表达水平均下调.结论:姜黄素具有一定的体外抗增生性瘢痕的作用.
Abstract
AIM: To evaluate the inhibitory effects of the curcumin against hypertrophic scar in vitro. METHODS: Hypertrophic scar fibroblasts ( HSFB), as target cells, were exposed to different concentrations of curcumin respectively. The proliferation of human HSFB was evaluated by methyl thiazolyl tetrazolium (MTT) colorimetric assay. The morphological characters of hypertrophic scar fibroblasts were analyzed by electron microscopy. The apoptosis induced by curcumin were studied by combination of flow cytometry (FCM) and Annexin V/Propidium Iodide double staining. The expressions of procollagen Ⅰ,procollagen Ⅲ and B-cell lymphoma/leukemia-2 (Bcl-2) in HSFB were detected by real-time PCR. RESULTS: Curcumin had a significant inhibitory effect on the proliferation of human HSFB. The number of apoptotic HSFB increased with elevation of the concentration of the curcumin, and these cells appeared typical ultrastructural features. The results of FCM indicated that the apoptosis of the HSFB in vitro was accelerated significantly by curcumin; Curcumin obviously inhibited the expression of the amount of type Ⅰ and Ⅲ procollagen, Bcl-2 mRNA of fibroblasts derived from hypertrophic scar. CONCLUSION: The curcumin inhibits hypertrophic scar in vitro to some extent.
关键词
姜黄素/增生性瘢痕/成纤维细胞/凋亡/Ⅰ型前胶原/Ⅲ型前胶原/Bcl-2Key words
curcumin/hypertrophic scar/fibroblast/apoptosis/procollagen Ⅰ/procollagen Ⅲ/Bcl-2引用本文复制引用
出版年
2009
NETL
NSTL
维普
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