Effect of constant magnetic field on myocardial ischemia-reperfusion injury and no-reflow area in rats
AIM:To investigate the effect of constant magnetic field (CMF) on myocardial ischemia/reperfusion injury ( IRI) and no-reflow area in rate. METHODS: Twenty-four male Spra-gue-Dawley rats were randomly divided into 4 groups: sham operation group, IRI group, 30 mT CMF intervention group and 100 mT CMF intervention group, with 6 in each group. The rats were anesthetized and then underwent 40 min of myocardial ischemia and 4 h of reperfusion. Size of no-reflow area was determined by thioflavin-S. The infarct size was assessed by Evans' blue and TTC staining. Level of sera cardiac troponin Ⅰ(cTn I) was detected by chemiluminescent enzyme immunoassay. Level of sera creatine kinase (CK) was determined by automatic biochemistry analyzer. RESULTS: The ratio of infarct size to area at risk (IS/ AAR) and size of no-reflow area decreased following intervention with 50 mT or 100 mT CMF, which were significantly smaller than those in IRI group ( P < 0. 05). Levels of sera CK and cTnI in IRI group were significantly higher than those in sham operation group (P <0.05), whereas levels of sera CK and cTnI in 50 mT or 100 mT CMF intervention group were significantly lower than those in IRI group (P<0. 05). CONCLUSION: Intervention with CMF could protect the heart against IRI and no-reflow.
constant magnetic fieldmyocardial ischemiareperfusion injuryno-reflow
NETL
NSTL
万方数据
维普
