Combination of chitosan and L-glutamine for treatment of gastric ulcer in rats
AIM: To observe anti-gastric ulcer activities by combining chitosan and L-glutamine in the rats. METHODS: The acute gastric ulcer was induced by intragastrical ethanol. The chronic gastric ulcer was induced by acetic acid. The ulcer tissues of stomach were collected to examine ulcer size and ulcer index. The volume of gastric juice, acidity, mucus secretion, and PGE2 were measured to elucidate the effects of chilosan and L-glutamine combination on treatment of gastric ulcer. RESULTS: The size of ulcer in chitosan and L-glutamine combination treatment group decreased more remarkably than that of chitosan or L-glutamine alone treatment group (ulcer index: combination,62. 4 ± 25. 6; chitosan, 98.2 ±26.9; L-glutamine, 105.3 ±35.4; n = 10, P< 0.05) in the same dosage. The mechanisms underlying anti-gastric ulcer involved reducing acidity [ NaOH volume ( mL) : model,1.8 ±0.3; chitosan, 1.3 ±0.3; L-glutamine, 1.6 ±0.3; combination, 1.1 ± 0. 2; n = 10, P <0. 05], increasing mucus secretion (model, 0.14 ±0.06; chitosan, 0.11 ±0.02; L-glutamine, 0. 12 ± 0. 03; combination, 0. 13 ± 0. 04; n = 10, P < 0.05) and PGE2 levels (model, 220.3 ±17.2; chitosan, 201.5 ±10. 2; L-glutamine, 198. 6 ± 9. 4; combination, 216. 3 ± 11.7; n= 10, P <0. 05) in the gastric mucus. However, the volume of gastric juice and pepsin activity were not changed. CONCLUSION: Combination of chitosan and L-glutamine displays more remarkable anti-gastric ulcer activities than that of chitosan or L-glutamine given alone, indicating a synergistic action between them.
NETL
NSTL
万方数据
维普
