新西兰兔烫伤模型的建立
Establishment of scalding model of New Zealand rabbit
刘利兵 1于军 1张学策 1周幸春 1铁茹1
作者信息
- 1. 第四军医大学教学实验中心,陕西,西安,710033
- 折叠
摘要
目的:建立一种简易的可控制面积及深度的烫伤模型.方法:以医用纱布作为热载体,确定可持续保温的最佳纱布层数.将恒温热水浴中加热至99℃的纱布分别在新西兰兔背部烫3,5,7,9,11,13,15,17,19,21,23,25 S共计12个时间点.伤后24 h取标本,经HE染色评价烫伤程度,并对烫伤的时间和程度作相关性分析.结果:确定可持续保温的纱布最佳层数为35层,并且烫伤程度和时间具有很好的相关性:Ⅰ度烫伤模型的致伤时间3～5 8;浅Ⅱ度烫伤模型的致伤时间7～13 8;深Ⅱ度烫伤模型的致伤时间15～19 8;Ⅲ度烫伤模型的致伤时间21～25 S.结论:该模型选用纱布作为热载体,具有烫伤面积及深度控制准确,操作简便、重复性好的优点,是研究创伤修复机制的较好模型.
Abstract
AIM: To establish a simple scalding model with controllable area and grades. METHODS: Pledgets were used as heating media. At first, the optimal layer numbers of pledgets were determined by evaluating the effect of heat preservation. Pledgets soaked with hot water at 99 ℃ were applied onto the backs of New Zealand rabbits for 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23 and 25 s respectively. After 24 h, samples were harvested and the scalding lesions were evaluated by HE staining. Also, the relationship between the heating time and scalding grades were statistically analyzed. RESULTS: The optimal layer numbers of pledgets proved to be 35. Importantly, there was a close relationship between the heating time and scalding grades. The time for preparing Ⅰ , superficial Ⅱ , deep II and Ⅲ grades scald was 3 - 5, 7 - 13, 15 - 19 and 21 - 25 s respectively. CONCLUSION: The current scalding model induced by pledgets as heating media boasts advantage of controllable areas and grades, easy manipulation and good reproducibility, which may be a valuable model to investigate the mechanism of repair in trauma.
关键词
新西兰兔/烫伤/动物模型Key words
New Zealand rabbits/scald/animal model引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据