Establishment of scalding model of New Zealand rabbit
AIM: To establish a simple scalding model with controllable area and grades. METHODS: Pledgets were used as heating media. At first, the optimal layer numbers of pledgets were determined by evaluating the effect of heat preservation. Pledgets soaked with hot water at 99 ℃ were applied onto the backs of New Zealand rabbits for 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23 and 25 s respectively. After 24 h, samples were harvested and the scalding lesions were evaluated by HE staining. Also, the relationship between the heating time and scalding grades were statistically analyzed. RESULTS: The optimal layer numbers of pledgets proved to be 35. Importantly, there was a close relationship between the heating time and scalding grades. The time for preparing Ⅰ , superficial Ⅱ , deep II and Ⅲ grades scald was 3 - 5, 7 - 13, 15 - 19 and 21 - 25 s respectively. CONCLUSION: The current scalding model induced by pledgets as heating media boasts advantage of controllable areas and grades, easy manipulation and good reproducibility, which may be a valuable model to investigate the mechanism of repair in trauma.
NETL
NSTL
万方数据
维普
