Effects of GnRHa on annexin 5 and 3β-HSD expression in rat Leydig cells
AIM: To investigate the effects of GnRHa on the expression of annexin5 and 3β-HSD in rat Leydig cells. METHODS: The primary Leydig cells were isolated, purified and cultured in vitro. The cells were identified by 3β-HSD immunocytochemistry method. Different concentrations of GnRHa (1 × 10 -5 , 1 × 10 -6, 1 × 10 -7 mol/L) were administrated on the Leydig cells for 24 hour. The changes of annexin5 and 3β-HSD expression were investigated by Western blot. RESULTS: GnRHa stimulated the annexin5 and 3β-HSD expression. Treatment with 10-5mol/L of GnRHa increased annexin5 expression by 69. 2% (P < 0. 01 ) and 3β-HSD expression by 25. 2% ( P < 0. 05). Treatment with 10 -6 mol/L and 10 -7 mol/L of GnRHa increased annexin5 expression by 61. 5% and 16. 64% (P > 0. 05 ) , and exerted no significant effects on 3β-HSD expression (increased by 7.7% and 2. 22% respectively, P > 0. 05). CONCLUSION: GnRHa can regulate the annexin5 and 3β-HSD expression in primary cultured Leydig cells.
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