卡维地洛对慢性心力衰竭大鼠心肌酪氨酸羟化酶表达的影响
Preventive effect of carvedilol on decrease of tyrosine hydroxylase expression in myocardium of rats with chronic heart failure
李书国 1曾秋棠 2杨俊 1丁家望 1李松 1吴辉 1李莉 1吕志阳1
作者信息
- 1. 宜昌市中心人民医院心内科,三峡大学第一临床医学院心内科,湖北宜昌,443000
- 2. 华中科技大学附属协和医院心内科,湖北武汉,430022
- 折叠
摘要
目的:评价卡维地洛(Carvedil01)对慢性心力衰竭(CHF)大鼠心肌酪氨酸羟化酶(TH)表达的影响.方法:应用阿霉素腹腔注射制作CHF大鼠模型,根据干预约物的不同随机分为Carvedilol治疗组(CHF-C组,n=9),倍他乐克(Betaloc)治疗组(CHF-M组,n=9),CHF安慰剂治疗组(CHF-P组,n=9)和正常埘照组(NC组,n=6).阿霉素应用第5周后采用灌胃给约途径,CHF-C组以Carvedilol 0.25 mg/kg,1次/d进行干预;CHF-M组以Betaloc 0.25 ms/kg,1次/d进行干预;CHF-P组和NC组加以食用淀粉0.25 ms/kg,1次/d进行干预.10 wk后透射电镜检测大鼠心肌超微结构,并采用RT-PCR和Western Blot方法检测大鼠心肌TH表达水平.结果:大鼠心肌TH mRNA和蛋白表达水平CHF-C组明显低于NC组[(0.94±0.31),us(1.18±0.39),(0.91±0.30)vs(1.32±0.41),均P<0.05];但明显高于CHF-P组[(0.94±0.31)vs(0.47±0.12),(0.91±0.30)vs(0.41±0.14),均P<0.05];也明显高于CHF-M组[(0.94±0.31)vs(0.66±0.20),(0.91±0.30)vs(0.58±0.19),均P<0.05].结论:CHF大鼠心肌TH表达水平明显下调,Carve-dilol治疗能够阻止CHF心肌TH下调,有助于改善CHF大鼠心肌交感神经重构.
Abstract
AIM: To investigate the effect of carvedilol on the expression of tyrosine hydroxylase( TH) in myocardium of chronic heart failure (CHF) rats. METHODS: Thirty-three adult male Wistar rats were randomly divided into 4 groups: CHF carvedilol administration group ( CHF-C group, n = 9 ) , CHF metoprolol administration group ( CHF-M group, n = 9), CHF placebo administration group ( CHF-P group, n =9) and normal control group(NC group, n =6). Adrimycin was injected intraperitoneal-ly to the rats of CHF groups to establish CHF models. Carvedilol was administrated intragastrically to rats in CHF-C group at a dose of 0.25 mg/ (kg · d) at the 5th week after adrimycin injection. Metoprolol was administrated intragastrically to rats in CHF-M group at a dose of 0. 25 mg/( kg · d) at the 5th week after adrimycin injection. Starch was administrated to rats in CHF-P and NC groups. TH mRNA expression in myocardial tissue was analyzed using the reverse transcriptase polymerase chain reaction, and TH protein expression in myocardial tissue was analyzed by Western blot. RESULTS: Compared those in NC group, the expressions of TH mRNA and protein in myocardium significantly decreased in CHF-C group[ (0.94 ±0.31) vs (1.18 ±0.39), (0.91 ±0.30) vs (1.32±0.41), P<0.05], Compared with those in CHF-P group, the expressions of TH mRNA and protein in myocardium significantly increased in CHF-C group [ (0. 94 ± 0.31) vs (0.47 ±0. 12), (0.91 ±0.30)vs (0.41 ±0. 14), P < 0.01 ]. The expressions of TH mRNA and protein in myocardium were not significantly different between CHF-M group and CHF-P group[ (0.66 ±0.20) vs (0.47 ±0.12) ,(0. 58 ±0. 19) vs (0.41 ± 0.14), P > 0.05 ]. CONCLUSION: The expression of TH is significantly decreased in CHF rats' myocardium. Carvedilol can prevent the decrease of TH expression in CHF rats' myocardium.
关键词
心力衰竭,充血性/卡维地洛/酪氨酸单氧化酶/心肌Key words
heart failure/congestive/tyrosine 3-monooxygen-ase/myocardium/carvedilol引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据