Effect of bile duct ligation induced biliary cholestasis on hepatocytes proliferation
AIM: To investigate the effect of biliary cholestasis on liver induced by bile duct ligation in mice. METHODS: Mice were subjected to bile duet ligation to establish cholestasis model. After biliary intervention, hepatic biochemical indicators in serum were detected with automatic biochemistry analyzer, hepatic pathological changes were observed with HE staining and cell proliferation related markers in liver were detected with immunohisto-chemistry. RESULTS: An obstructive cholestasis mouse model was successfully established. Serum biochemical and pathological changes indicated impaired liver function in bile duct-ligated mice. After cholestasis caused by bile duct ligation, mitotic phase in hepatocytes and the expression of proliferation markers in hepatocytes and biliary epithelial cells increased, indicating that prolif-erative activity of liver cells increased significantly after obstructive cholestasis. CONCLUSION: Cholestasis induced by bile duct ligation promotes the proliferation of liver cells.
common bile ductligationcholestasiscell proliferation
NETL
NSTL
万方数据
维普
