胆总管结扎引起胆汁淤积对小鼠肝脏细胞增殖的影响
Effect of bile duct ligation induced biliary cholestasis on hepatocytes proliferation
罗丹 1文阳安 1董玉芳 1周倩云 1张莉萍1
作者信息
- 1. 重庆医科大学附属第一医院检验科,重庆400016
- 折叠
摘要
目的:探讨胆总管结扎引起的胆汁淤积对小鼠肝脏增殖的影响.方法:采用胆总管结扎手术制备胆汁淤积小鼠模型;利用全自动生化分析仪检测小鼠血清肝脏生化指标水平;用免疫组织化学方法检测小鼠肝脏中增殖相关的指标的表达情况.结果:通过胆总管结扎手术成功制备了梗阻性胆汁淤积小鼠模型;小鼠血清生化指标以及病理组织学变化表明小鼠肝脏功能受损,胆总管结扎手术成功;胆总管结扎引起胆汁淤积后,肝脏细胞有丝分裂相增加,肝细胞和胆管细胞中增殖相关的标志物表达升高,表明细胞增殖活性增强.结论:胆总管结扎引起的胆汁淤积可以引起肝脏细胞以及胆管上皮细胞的增殖.
Abstract
AIM: To investigate the effect of biliary cholestasis on liver induced by bile duct ligation in mice. METHODS: Mice were subjected to bile duet ligation to establish cholestasis model. After biliary intervention, hepatic biochemical indicators in serum were detected with automatic biochemistry analyzer, hepatic pathological changes were observed with HE staining and cell proliferation related markers in liver were detected with immunohisto-chemistry. RESULTS: An obstructive cholestasis mouse model was successfully established. Serum biochemical and pathological changes indicated impaired liver function in bile duct-ligated mice. After cholestasis caused by bile duct ligation, mitotic phase in hepatocytes and the expression of proliferation markers in hepatocytes and biliary epithelial cells increased, indicating that prolif-erative activity of liver cells increased significantly after obstructive cholestasis. CONCLUSION: Cholestasis induced by bile duct ligation promotes the proliferation of liver cells.
关键词
胆总管/结扎术/胆汁淤积/细胞增殖Key words
common bile duct/ligation/cholestasis/cell proliferation引用本文复制引用
基金项目
国家自然科学基金(30670965)
教育部重点研究课题(207099)
重庆市科学技术委员会科研基金(2006BB5030)
出版年
2009
NETL
NSTL
维普
万方数据