Establishment and evaluation of rabbit model of femoral head avascular necrosis
AIM: To establish an ideal animal model for the study of avascular necrosis of the femoral head( ANFH) disease. METHODS: Twenty-four healthy New Zealand white rabbits were randomly divided into 3 groups: horse serum and dexametha-sone group(A, n = 10), liquid nitrogen frozen group(B, n = 10) and control group(C, n =4). X-ray and histological examination were performed periodically after experiment to evaluate the osteo-necrosis. RESULTS: Necrosis of the femoral head at 4 th and 8 th week was confirmed in A and B groups, in both of which the empty bone lacuna rate was higher than that in group C. The success rate was less than 55% after the 8th week in group A, while it was more than 95% in the group B. CONCLUSION: The model of ANFH disease can be built up by steroid and serum injection or liquid nitrogen in rabbits, but not all the rabbits model can be built by steroid and serum injection. The animal model of ANFH disease can be established satisfactorily with liquid nitrogen freezing.
femur head necrosisliquid nitrogenfreezingrabbitsdisease modelsanimal
NETL
NSTL
万方数据
维普
