In vitro specific anti-chronic myeloid leukemia cell effect of CTL induced by a multiple epitope BCR-ABL fusion protein
AIM: To assay the anti-leukemia effect of cytotoxic lymphocytes(CTLs) induced by a multiple epitope BCR-ABL fusion protein in vitro acquired by gene engineering technology so as to explore a new method of immunotherapy for chronic myeloid leukemia. METHODS: Dendritic cells ( DCs) were generated from peripheral mononuclear cells by co-cultivation with granulo-cyte/macrophage-colony stimulating factor ( GM-CSF) and inter-leukin-4(IL-4). Pulsed with the BCR-ABL fusion protein antigen, the harvested DCs were transformed into specific CTLs. The specific lytic activities of CTLs on target cells were detected by standard MTT assay. RESULTS: CTLs stimulated with the fusion protein inhibited the growth of b3a2 + target cells(P <0.05) , including K562 cells( P <0.01) and HLA-A2 +/b3a2 + CML cells ( P < 0. 05), but exerted no effect on HLA-A2-or b2a2 + target cells. CONCLUSION: The multiple epitope BCR-ABL fusion antigen in this study can induce specific anti-CML immunological reaction and inhibit the growth of b3a2 + leukemia cells in vitro, which may provide a research basis for further investigation on the strategy of immunotherapy for CML in vivo.
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万方数据
维普
