白藜芦醇对人宫颈癌SiHa细胞增殖与凋亡作用的影响
Effect of Resveratrol on proliferation and apoptosis of human cervical cancer cell Line SiHa
申新 1于良 2张靖 3吕淑兰 3潘承恩2
作者信息
- 1. 西安交通大学医学院第一附属医院麻醉科,陕西西安,710061
- 2. 西安交通大学医学院第一附属医院肝胆外科,陕西西安,710061
- 3. 西安交通大学医学院第一附属医院妇产科,陕西西安,710061
- 折叠
摘要
目的:探讨白藜芦醇(Res)诱导宫颈癌SiHa细胞凋亡的作用及可能的机制.方法:MTT法检测不同浓度(0,12.5,25,50,100,200,300,400和600μmol/L)Res处理24,48和72 h对SiHa细胞的抑制率;流式细胞仪采用Annexln V和PI双染检测细胞的凋亡率.荧光显微镜观测SiHa细胞的形态学改变.分光光度法检测Caspase-3活性.结果:Res在一定浓度范围内,以浓度和时间依赖的方式抑制宫颈癌SiHa细胞的生长(P<0.05).以0,200和300 μmol/L Res处理细胞48 h,SiHa细胞早期凋亡率分别为1.1%,8.9%和11.1%,晚期凋亡比例为3.1%,15.0%和14.0%.荧光显微镜下细胞呈现典型的凋亡性改变.200 μmol/L Res处理8 h后,Caspase-3活性增加,24 h达高峰,后逐渐下降,经50,100和200 μmol/L Res处理24 h后,Caspase-3活性与对照组相比,分别增加了1.92倍,2.51倍和4.53倍(P<0.05).结论:白藜芦醇通过诱导Caspase-3活性增加以时间依赖和浓度依赖的方式抑制宫颈癌SiHa细胞增殖,诱导细胞凋亡.
Abstract
AIM: To explore the effect of resveratrol(Res) on the apoptosis of cervical cancer SiHa cells and its related mechanism. METHODS: SiHa cells were cultured and treated with different concentrations(0,12.5,25,50,100,200,300,400,600 μmol/L) of Res for 24, 48 and 72 h. The growth inhibition rate of SiHa cells was detected by MTT method, and cell apoptosis rate was analyzed by flow cytometry ( FCM) using annexinV/pro-pidium iodide(PI) double staining. Morphologic changes of apop-totic cells were observed by fluorescence microscope and caspase-3 activity was assessed by colorimetric assay. RESULTS: Res inhibited the proliferation of SiHa cells in a time-and dose-dependent manner( P < 0. 05). After Res treatment at 0, 200 and 300 μmol/L for 48 h, the apoptosis rate of SiHa cells was 1.1%, 8.9% and 11. 1%, respectively, while the necrosis rate was 3.1%, 15. 0% and 14. 0%, respectively. The cells showed characteristic apoptotic morphologic changes under fluorescence microscope. After 200 μmol/L Res treatment for 8 h, the activity of caspase-3 increased, reached the peak at the 24 h and then declined gradually. After SiHa cells were treated with 50,100 and 200 μmol/L Res for 24 h, the activity of caspase-3 was 1. 92, 2.51 and 4.53 folds as high as that in control group(P <0.05). CONCLUSION: Res inhibits the proliferation and induces the apoptosis of SiHa cells in a time-and dose-dependent manner by increasing caspase-3 activity.
关键词
白藜芦醇/宫颈肿瘤/细胞增殖/细胞凋亡/caspase-3Key words
resveratrol/cervix neoplasms/cell proliferation/apoptosis/caspase-3引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据