Effect of Resveratrol on proliferation and apoptosis of human cervical cancer cell Line SiHa
AIM: To explore the effect of resveratrol(Res) on the apoptosis of cervical cancer SiHa cells and its related mechanism. METHODS: SiHa cells were cultured and treated with different concentrations(0,12.5,25,50,100,200,300,400,600 μmol/L) of Res for 24, 48 and 72 h. The growth inhibition rate of SiHa cells was detected by MTT method, and cell apoptosis rate was analyzed by flow cytometry ( FCM) using annexinV/pro-pidium iodide(PI) double staining. Morphologic changes of apop-totic cells were observed by fluorescence microscope and caspase-3 activity was assessed by colorimetric assay. RESULTS: Res inhibited the proliferation of SiHa cells in a time-and dose-dependent manner( P < 0. 05). After Res treatment at 0, 200 and 300 μmol/L for 48 h, the apoptosis rate of SiHa cells was 1.1%, 8.9% and 11. 1%, respectively, while the necrosis rate was 3.1%, 15. 0% and 14. 0%, respectively. The cells showed characteristic apoptotic morphologic changes under fluorescence microscope. After 200 μmol/L Res treatment for 8 h, the activity of caspase-3 increased, reached the peak at the 24 h and then declined gradually. After SiHa cells were treated with 50,100 and 200 μmol/L Res for 24 h, the activity of caspase-3 was 1. 92, 2.51 and 4.53 folds as high as that in control group(P <0.05). CONCLUSION: Res inhibits the proliferation and induces the apoptosis of SiHa cells in a time-and dose-dependent manner by increasing caspase-3 activity.
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万方数据
维普
