Effects of angiopoietin-1 on rhesus macaque choroids-retinal endothelial cells monolayer permeability in high glucose environment
AIM: To explore the effects of high concentration glucose and angiopoietin-1 (Ang-1) on monolayer permeability of rhesus macaque choroids-retinal endothelial cells ( ECs). METHODS: Confluent ECs monolayer was developed on polycarbonate micropore filter. The cells were divided into 3 groups: control group (DMEM containing 25 mmol/L glucose), high glucose group (DMEM containing 30 mmol/L glucose) and Ang-1 + high glucose group (DMEM containing both 30 mmol/L glucose and 0. 25 mg/L Ang-1 ). The ECs were perfused with plain D-Hank's balanced salt solution containing 1 g/L albumin to detect the fluid filtration coefficient ( Kf) and osmotic inflation coefficient (δ) to the albumin at the 1st, 3rd, 5th and 7th day, respectively. Additionally, DNA agarose gel electrophoresis was performed at the 5th and 7th day, respectively. RESULTS: The monolayer permeability of ECs in the high glucose group was higher than that in control group at the 3rd, 5th and 7th day (P < 0.01). But no significant difference was observed in the permeability between control group and Ang-glucose group (P > 0.05). DNA ladder was only presented in high glucose group at the 5th and 7th day. CONCLUSION: High concentration glucose increases the EC monolayer permeability by accelerating the speed of apoptosis of ECs. Ang-1 may prevent ECs from apoptosis and oppose the effects of high glucose on EC monolayer permeability.
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维普
