养阴抗毒胶囊对阴虚大鼠肾上腺皮质细胞凋亡及TNF-α的影响
Influence of Nourishing Yin Antitoxic Capsule on adrenocortical cell apoptosis and TNF-α in Yin deficiency rats induced by hydrocortisone
史恒军 1侯颖 1朱云 2李宁 1张野 3任秦有1
作者信息
- 1. 第四军医大学唐都医院,中医科,陕西,西安,710038
- 2. 解放军第302医院肝癌治疗中心,北京,100039
- 3. 第四军医大学唐都医院,感染病院,陕西,西安,710038
- 折叠
摘要
目的:研究养阴抗毒胶囊(YC)拮抗糖皮质激素副作用,保护肾上腺皮质功能的机制.方法:将30只大鼠分为对照组、氢化可的松阴虚模型组和YC治疗组,用末端脱氧核苷酸转移酶介导缺口末端标记法(TUNEL)原位检测肾上腺皮质细胞凋亡;用RT-PCR法检测肾上腺组织细胞因子TNF-α的表达水平.结果:YC组细胞凋亡率高于正常对照组但低于阴虚模型组;阴虚模型组TNF-α水平升高,YC治疗后可使其水平下降.结论:养阴抗毒胶囊可减少氢化可的松所致"阴虚"证大鼠细胞凋亡,降低TNF-α的表达水平,是YC保护肾上腺皮质功能的机制之一.
Abstract
AIM: To identify the underlying mechanisms of adrenal function recovery by Nourishing Yin Antitoxic Capsule (YC) in Yin-deficiency syndrome rats. METHODS: Thirty rats were separated into three groups: control group, hydrocortisone model group and YC treatment group. TUNEL was employed to display apopotic cells in situ. The level of TNF-α was measured by RT-PCR. RESULTS: Compared to control group, hydrocortisone group had increased apoptotic rate and level of TNF-α, which were reversed by YC. CONCLUSION: YC inhibits the adrenocortical cell apoptosis and the decrease of TNF-α level induced by hydrocortisone, which contributes to adrenal function recovery.
关键词
养阴抗毒胶囊/糖皮质激素/阴虚证/细胞凋亡/肿瘤坏死因子α/大鼠Key words
nourishing Yin Antitoxic Capsule/glueocorticoids/Yin-deficiency syndrome/apopotic/TNF-α/rat引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据