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扫描电镜观察高功率微波辐照后大鼠小肠的形态学改变

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目的:利用扫描电镜观察高功率微波(HPM)辐照后大鼠小肠形态结构的变化.方法:雄性SD大鼠35只随机分为对照组和试验组,在源距4.5m,场强100 kV/m,总脉冲数40万次微波辐射后3,24,72h及7d活杀大鼠,取小肠组织;甲醛固定制备光镜标本;30mL/L戊二醛固定,进行常规扫描电镜标本制备.结果:扫描电镜观察对照组和HPM辐照后3h小肠形态基本正常,小肠绒毛排列整齐.辐照后24~72h,主要以肠黏膜破坏、肠上皮细胞水肿和分泌物增多为主.辐照后7d,分泌物减少,肠绒毛水肿减轻.结论:说明HPM辐照后扫描电镜观察大鼠小肠随作用时间的不同有不同程度的损伤.
Morphologic changes of small intestines in rats after exposure to high power microwave observed with scanning electron microscope
AIM: To observe the morphologic changes of small intestines in rats after exposure to high power microwave (HPM). METHODS: Thirty - five male SD rats were divided into control and experimental groups. The rats in experimental group were exposed to HPM at 100 kv/m for 4 × 105 pulses. At different time points (3 h, 24 h, 72 h and 7 d) after exposure to HPM, rats were sacrificed. The samples were fixed in 30 g/L glutaralde-hyde. The morphology of small intestines was observed under scanning electron microscope (SEM). RESULTS: At 3 h after exposure to HPM, small intestinal villi arranged regularly as those in control group. At 24 - 72 h after exposure to HPM, destroyed intestinal mucosa, swelled intestinal columnar epithelium and increased secretion were observed. At 7 d after exposure to HPM, secretion and edema of the intestinal villi reduced. CONCLUSION : Scanning electron microscopy demonstrates that high power microwave exposure at different time may cause different degrees of damage in the morphologic structures in rats.

small intestinescanning electron microscopyrathigh power microwave

亢君君、王春梅、杨家骥、黄晓峰、尹文、于华、王爽、孟瑞、李永强

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第四军医大学基础部中心实验室,陕西,西安,710033

小肠组织 扫描电镜 大鼠 高功率微波

2009

第四军医大学学报
第四军医大学

第四军医大学学报

CSTPCDCSCD北大核心
影响因子:0.599
ISSN:1000-2790
年,卷(期):2009.30(3)
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