扫描电镜观察高功率微波辐照后大鼠小肠的形态学改变
Morphologic changes of small intestines in rats after exposure to high power microwave observed with scanning electron microscope
亢君君 1王春梅 1杨家骥 1黄晓峰 1尹文 1于华 1王爽 1孟瑞 1李永强1
作者信息
- 1. 第四军医大学基础部中心实验室,陕西,西安,710033
- 折叠
摘要
目的:利用扫描电镜观察高功率微波(HPM)辐照后大鼠小肠形态结构的变化.方法:雄性SD大鼠35只随机分为对照组和试验组,在源距4.5m,场强100 kV/m,总脉冲数40万次微波辐射后3,24,72h及7d活杀大鼠,取小肠组织;甲醛固定制备光镜标本;30mL/L戊二醛固定,进行常规扫描电镜标本制备.结果:扫描电镜观察对照组和HPM辐照后3h小肠形态基本正常,小肠绒毛排列整齐.辐照后24～72h,主要以肠黏膜破坏、肠上皮细胞水肿和分泌物增多为主.辐照后7d,分泌物减少,肠绒毛水肿减轻.结论:说明HPM辐照后扫描电镜观察大鼠小肠随作用时间的不同有不同程度的损伤.
Abstract
AIM: To observe the morphologic changes of small intestines in rats after exposure to high power microwave (HPM). METHODS: Thirty - five male SD rats were divided into control and experimental groups. The rats in experimental group were exposed to HPM at 100 kv/m for 4 × 105 pulses. At different time points (3 h, 24 h, 72 h and 7 d) after exposure to HPM, rats were sacrificed. The samples were fixed in 30 g/L glutaralde-hyde. The morphology of small intestines was observed under scanning electron microscope (SEM). RESULTS: At 3 h after exposure to HPM, small intestinal villi arranged regularly as those in control group. At 24 - 72 h after exposure to HPM, destroyed intestinal mucosa, swelled intestinal columnar epithelium and increased secretion were observed. At 7 d after exposure to HPM, secretion and edema of the intestinal villi reduced. CONCLUSION : Scanning electron microscopy demonstrates that high power microwave exposure at different time may cause different degrees of damage in the morphologic structures in rats.
关键词
小肠组织/扫描电镜/大鼠/高功率微波Key words
small intestine/scanning electron microscopy/rat/high power microwave引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据