摘要
目的:观察肠功能恢复汤、透明质酸钠及两药合用预防大鼠腹膜粘连的效果及对粘连组织IL-1β,TGF-β1的影响.方法:选用SD大鼠85只,随机分为5 组:空白对照组(n=5)、模型对照组(n=20)、透明质酸钠组(n=20)、肠功能恢复汤组(n=20)、透明质酸钠与肠功能恢复汤合用组(n=20).除空白对照组外,其余组大鼠均按Ellis法制备成腹膜粘连模型,术后第1,3,5日取粘连或损伤处组织;于术后第7日取所有组的粘连或损伤处组织,免疫组化法测定IL一1β及TGF-β1,表达水平,进行粘连程度肉眼分级并做病理检查.结果:模型对照组粘连分级明显高于其余各组(P<0.05).模型对照组的IL-1β,TGF-β1,水平明显高于空白对照组(P均<0.001);与模型对照组相比,透明质酸钠组、肠功能恢复汤组及透明质酸钠与肠功能恢复汤合用组的IL-1β,TGF-β1.含量均显著降低(P均<0.05).结论:透明质酸钠、肠功能恢复汤及透明质酸钠与肠功能恢复汤合用能降低腹膜粘连大鼠粘连组织中IL-1β,TGF-β1的含量,尤以两药合用效果最佳.细胞因子IL-1β,TGF-β1,在腹膜粘连中成中发挥重要作用.
Abstract
AIM: To investigate the effect of intestinal function recovering decoction or sodium hyaluronate, and the combination of the two on peritoneal adhesion and on IL-1 β and TGF-β1 in rats with peritoneal adhesion. METHODS: Eighty-five SD rats were randomly divided into five groups: control group ( n = 5), model group (n = 20), sodium hyaluronate group (n =20), decoction group ( n = 20) and combination group (n = 20). Peritoneal adhesion models were established with Ellis method in all the rats except those in the control group. The adhesion or injured tissue was collectd on day 1,3,5 and 7 postoperatively. The levels of IL-1β and TGF-β1 were determined immunohistochemically. Visual adhesion gradation and pathological examination were also performed. RESULTS: The adhesion grade in model group was significantly higher than that in the other 4 groups (P < 0. 05). The levels of IL-1 β and TGF-β1 were significantly higher in model group than those in control group ( P < 0.001). Compared with those in model group, the levels of IL-1 β and TGF-β1 in sodium hyaluronate, decoction and combination groups were significantly reduced (P<0.05). CONCLUSION: Sodium hyaluronate, intestinal function recovering decoction and the combination of the two drugs all reduce the levels of IL-1 β and TGF-β1 in rats with peritoneal adhesion, but the combined use of the two drugs achieves the best effect. The cytokines IL-1 β and TGF-β1 play some important roles in adhesion formation.
NETL
NSTL
维普
万方数据