Influence of intestinal function recovering decoction combined with sodium hyaluronate on IL-1β,TGF-β1 in rat with peritoneal adhesion
AIM: To investigate the effect of intestinal function recovering decoction or sodium hyaluronate, and the combination of the two on peritoneal adhesion and on IL-1 β and TGF-β1 in rats with peritoneal adhesion. METHODS: Eighty-five SD rats were randomly divided into five groups: control group ( n = 5), model group (n = 20), sodium hyaluronate group (n =20), decoction group ( n = 20) and combination group (n = 20). Peritoneal adhesion models were established with Ellis method in all the rats except those in the control group. The adhesion or injured tissue was collectd on day 1,3,5 and 7 postoperatively. The levels of IL-1β and TGF-β1 were determined immunohistochemically. Visual adhesion gradation and pathological examination were also performed. RESULTS: The adhesion grade in model group was significantly higher than that in the other 4 groups (P < 0. 05). The levels of IL-1 β and TGF-β1 were significantly higher in model group than those in control group ( P < 0.001). Compared with those in model group, the levels of IL-1 β and TGF-β1 in sodium hyaluronate, decoction and combination groups were significantly reduced (P<0.05). CONCLUSION: Sodium hyaluronate, intestinal function recovering decoction and the combination of the two drugs all reduce the levels of IL-1 β and TGF-β1 in rats with peritoneal adhesion, but the combined use of the two drugs achieves the best effect. The cytokines IL-1 β and TGF-β1 play some important roles in adhesion formation.
NETL
NSTL
万方数据
维普
