Expression and purification of BCSP31 protein of Brucella melitensis and preparation of monoclonal antibodies against BCSP31
AIM: To express and purify BCSP31 protein from Brucella melitensis and prepare monoclonal antibodies ( mAb) against it. METHODS: The mAb against BCSP31 was made by routine procedures: purifying protein via GST affinity chromatog-raphy, immunizing mice with purified reeombinant protein, fusing cells, cloning by indirect ELISA, preparing ascites and purifying ascites by caprylic acid and ammonium sulfate. The characteristics of the mAb were analyzed by Western Blot and ELISA. RESULTS: The fusion protein GST-BCSP31 was expressed in soluble form at 25℃. The purified BCSP31 protein 0.5 g/L was obtained and was reactive with the positive serum from Brucella melitensis immunized rabbits. Two mAb strains, named 1F1 and 1E5, were obtained. CONCLUSION: Purified BCSP31 protein and its mAb may play some important roles in the diagnosis and prevention of Brucellosis.
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万方数据
维普
