Effect of adipose-derived stem cells transplantation on motor function in intracerebral hemorrhage rats
AIM: To explore the effect of adipose-derived stem cells ( ADSCs) transplantation on motor function in intracerebral hemorrhage rate. METHODS: ADSCs were cultured and proliferated in vitro. After the rats underwent intracerebral hemorrhage for 24 h, ADSCs were stereotaxically transplanted into the right lateral ventricles. The motor function was evaluated at 3, 7, 14, 21 and 28 d after transplantation. Rats in each group were euthanized on time and brain sections were made. Immunohistochernis-try was employed to observe the distribution of transplanted cells and TUNEL assay was conducted for apoptosis detection. RESULTS : The transplanted cells migrated gradually from ventricles to the vicinities of hemorrhage focus and the hole of needle. The majority of apoptotic cells distributed around the focus of hemorrhage. Compared with the control, ADSCs-transplanted group had better motor function at 7, 14, 21 and 28 d (P < 0.05) and fewer apoptotic cells at 3, 7, 14, 21 and 28 d after transplantation (P<0. 05). CONCLUSION: ADSCs transplantation may improve the motor function by reducing apoptotic cells in intracerebral hemorrhage rats.
NETL
NSTL
万方数据
维普
