腺病毒介导IL-24基因对U251胶质瘤细胞p38 MAPK及bcl-2表达的影响
Effect of adenovinls-mediated IL-24 gene on expression of p38 MAPK and bcl-2 in U251 glioma cells
韩硕 1李文玲 2赵俊霞 1王彦玲 1曹翠丽 3闫蕴力1
作者信息
- 1. 河北医科大学,细胞生物学研究室,河北,石家庄,050017
- 2. 河北省人民医院功能神经外科,河北,石家庄,050051
- 3. 河北医科大学,人体解剖学教研室,河北,石家庄,050017
- 折叠
摘要
目的:探讨腺病毒介导的IL-24基因(AdSF35-hIL-24)对人胶质瘤细胞(U251细胞)的杀伤作用,以及对p38丝裂原活化蛋白激酶(p38 MAPK)和bel-2蛋白表达的影响.方法:应用MTT方法和双荧光染色方法测定Ad5F35-hIL-24对U251细胞的抑制作用;蛋白质印迹法检测p38 MAPK,p-p38MAPK和bcl-2的表达变化.结果:M1T和双荧光染色方法检测表明,与空载体Ad5F35-VEC组及空白对照组相比,Ad5F35-hIL-24对U251细胞有明显抑制作用.蛋白质印迹检测显示,Ad5F35-hIL-24组的p-p38 MAPK蛋白表达水平增加,bcl-2蛋白表达减少.结论:腺病毒介导的1L-24基因对胶质瘤U251细胞杀伤作用明显,提示p38 MAPK信号转导通路及bcl-2基因表达是Ad5F35-hIL-24重要作用靶点,该结果对于临床治疗胶质瘤有一定参考意义.
Abstract
AIM: To investigate the killing effects of Ad5F35-hIL-24 on U251 cells and its effect on p38 mitogen-activated protein kinase ( MAPK) pathway and bcl-2 protein expression. METHODS: Methyl thiazolyl tetrazolium (MTT) and double fluorescence staining were used to analyze the inhibition rate of U251 cells. Western Blot was applied to detect the protein expression of p38 MAPK, p-p38 MAPK and bcl-2. RESULTS: Ad5F3S-hIL-24 inhibited U251 cells in a dose-dependent manner. Ad5F35-hIL-24 increased p-p38 MAPK protein expression and decreased bcl-2 protein expression compared with those in control groups. CONCLUSION: p38 MAPK and bcl-2 play some important roles in IL-24 gene induced U251 cell apoptosis. These results are of some value for clinical treatment of glioma.
关键词
白介素-24基因/p38丝裂原活化蛋白激酶/bcl-2基因/胶质瘤Key words
IL-24 gene/p38 MAPK/bcl-2/glioma引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据