AIM: To search for a method to prepare a swine-mouse hybridoma. METHODS: Healthy swines were immunized with classical swine fever virus ( CSFV) by intramuscular injection to induce anti-CSFV antibody. B cells of the immunized swine were fused with mouse myeloma cells NSO and swine-mouse hybridoma was selected by CSFV and E2 protein. Neutralization test was carried out to identify the neutralizing titers of the swine antibodies. RESULTS: Fifteen strains of anti-CSFV swine-mouse monoclonal antibodies (mAb) were obtained after several times of screening and cloning, 9 of which were neutralizing antibodies and were recognized by recombinant E2 protein. CONCLUSION: The swine mAb may provide a valuable basis for further research of immunologic mechanism of CSFV in swines.
NETL
NSTL
万方数据
维普
