骨水泥复合材料制片法的研究
Investigation of the section techniques of bone cement composite materials
文艳华 1裘秀春 1周勇 1张殿忠 1李红 1尹艳 1刘云燕 1范清宇1
作者信息
- 1. 第四军医大学唐都医院骨科,陕西,西安,710038
- 折叠
摘要
目的:研究骨水泥复合材料标本的制片方法,为骨科和口腔科学实验提供需求.方法:将修复骨缺损的骨水泥复合材料标本离体后,锯成或磨成1cm3或8cm3大小,标本立即固定于BSSF液中,进行脱钙、脱水,用石蜡包埋切片,HE染色显微镜观察.结果:研制的骨水泥复合材料标本达到满意效果并能够顺利切片,经HE染色和三色染色观察骨组织、材料、新生骨组织结构良好,能满足临床及基础实验需要.结论:我们研制的骨水泥复合组织制片方法操作简单、结果可靠,基本达到适应骨科和口腔科学实验要求.
Abstract
AIM: To investigate the section techniques of specimens using bone cement composite materials. METHODS: The specimens of cement composite materials, taken out from patient's body, were sawed or ground into small pieces sized 1-8 cm3. These specimen pieces were immediately fixed in the solution BSSF to be demineralized, dehydrated, embedded with paraffin, sectioned, HE stained and finally microchecked. RESULTS: The specimens were successfully sectioned using this technique. After He-staining and trichrome-staining, the bone tissues and newly formed bone tissues the section could be satisfactorily viewed. CONCLUSION: Our method is simple with reliable results , which can basically meet the requirements of experiments in orthopedics.
关键词
骨水泥/复合材料/标本制备/复合组织双重胶接磨片Key words
bone cement/composite material/specimen handling/complex tissue double glued joint ground section引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据