Sequencing and analysis of rDNA-ITS2 Taenia in regions around Erhai in Yunnan
AIM: To sequence rDNA - ITS2 Taenia sampled from Erhai in Yunnan to determine the distribution of Taenia asiatica in Erhai regions and to explore the taxonomic status of Taenia asiatica. METHODS: Adult Taenia saginata were sampled from Wase, Eryuan, Xizhou, Zhoucheng and Shuanglang in Dali, Yunnan Province as well as Duyun and Congjiang in Guizhou Province. DNA was extracted from the samples as well as traditional Taenia saginata and then rDNA - ITS2 region was amplified for sequencing. A phylogenetic tree was constructed based on the sequencing results. RESULTS: According to the phylogenetic tree by ITS2 sequences, the samples from Eryuan, Wase and Congjiang clustered, and those from Zhoucheng, Xizhou, Shuanglang and Duyun clustered. CONCLUSIONS: There are Taenia saginata in Eryuan and Wase, and Taenia asiatica in Zhoucheng, Xizhou and Shuanglang. Overlapping exists in geographical distribution of Taenia saginata and Taenia asiatica.
NETL
NSTL
万方数据
维普
