大黄素在小鼠皮肤移植中的免疫抑制作用的实验研究
Immunosuppressive effects of emodin on skin transplantation in mice
刘原兴 1沈乃营 1刘昌 1吕毅1
作者信息
- 1. 西安交通大学医学院第一附属医院肝胆外科,陕西西安,710061
- 折叠
摘要
目的:研究大黄素对小鼠皮肤移植的免疫抑制作用,探讨其町能的作用机制.方法:建立小鼠C57BL/6→BALB/c异体皮肤移植模型.以大黄素和环孢素A(CsA)分别灌胃,观察不同药物及药物浓度对小鼠皮肤移植物存活时问的影响.采用ELISA法检测大黄索和CsA对皮肤移植小鼠血浆IL-2表达水平的影响.组织学观察不同药物浓度对移植物病理变化的影响.结果:大黄素显著降低小鼠血浆内IL-2的浓度,同时伴有移植物的存活时间的延长.组织学观察发现大黄素可以减轻移植排斥反应的强度,其作用表现出量效依赖性,并与CsA具有协同性.结论:大黄素能够减轻C57BL/6→BALB/c异体皮肤移植的排斥反应强度,其作用机制可能与抑制IL-2的分泌有关.
Abstract
AIM: To investigate the immunosuppressive effects of emodin on skin transplantation in mice and its immunosuppres-sive mechanism. METHODS: C57BL/6→BALB/c skin transplantation models were established and then treated with different concentrations of emodin and cyclosporine A (CsA ). The different mean survival times ( MST) of different groups were observed. Changes of plasma cytokines (IL-2) in mouse skin transplantation models were determined by ELISA and histological changes at different concentrations of emodin and cyclosporine A ( CsA ) were investigated. RESULTS: In the group treated with emodin, the MST of skin graft was prolonged and production of plasma IL-2 decreased. The histological results suggested that emodin relieved the graft immunological damage in a dose-dependent manner and exerted synergistic effect with CsA. CONCLUSION: Emodin exerts some immunosuppressive effects on mouse skin transplantation possibly by suppressing IL-2.
关键词
大黄素/皮肤移植/白细胞介素2/免疫抑制/小鼠Key words
Emodin/skin transplantation/IL-2/imraunosuppres-sive effect/mouse引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据