Empirical study of neural stem cell transplantation for spinal injury
AIM; To observe the effects of neural stem cells (NSCs) transplantation on the function recovery of rats with spinal cord injury. METHODS: Thirty Wistar rate were randomly divided into three groups: control group, injury group and NSCs transplantation group, with 10 rats in each group. The models of L4 plane with full transection of spinal cord were made in the rats in injury group and NSCs transplantation group. The cultured NSCs suspension of rats was injected into the injured spinal cord in transplantation group while equal volume saline was injected into the rats in injury group. Two months after operation, the motor and conduction functions of the spinal cord were determined using BBB scores cortical somatosensory evoked potential (CSEP) and horseradish peroxidase (HRP) retrograde tracing. RESULTS: By BBB scores, recovery was observed in the rats in injury group and NSCs transplantation group, but not to the normal level. The recovery in NSCs transplantation group was significantly better than that in injury group. CSEP in both injury group and transplantation group disappeared. The waveform in transplantation group was recovered and the latency tune of CSEP in NSCs transplantation group was prolonged. HRP labeled neurons were found in the anterior horn of the spinal cord in the control group but no neuron was found in the injury group. Some HRP labeled neurons were found in the NSCs transplantation group, but the number of HRP labeled neurons was fewer than that in control group. CONCLUSION: NSCs transplantation improves the partial recovery of motor and conduction functions of injured spinal cord in rats.
NETL
NSTL
万方数据
维普
