摘要
目的:观察神经干细胞(NSCs)移植对脊髓损伤(SCl)大鼠功能恢复的作用.方法:30只Wistar大鼠随机分为对照组、损伤组和移植组,每组10只;损伤组和移植组制作成k平面的脊髓全横断模型,将培养的大鼠NSCs悬液注入移植组损伤脊髓处,损伤组注射等鼍的生理盐水.术后2 mo,采用BBB评分、皮层体感诱发电位(CSEP)和辣根过氧化物酶(HRP)逆行示踪技术观察大鼠脊髓运动和传导功能的恢复程度.结果:术后2 mo,BBB评分损伤组、移植组大鼠有所恢复,但都未达到正常水平,其中移植组的大鼠恢复较好,评分较高,与损伤组有统计学差异.SCI后,损伤组、移植组的CSEP波消失,术后2 mo移植组的波形有所恢复,但潜伏期延长.对照组脊髓前角可见到许多HRP标记阳性神经元,损伤组未见阳性神经元,移植组可见有阳性神经元,但数目较对照组少.结论:NSCs脊髓内移植能促进损伤脊髓运动和传导功能的部分恢复.
Abstract
AIM; To observe the effects of neural stem cells (NSCs) transplantation on the function recovery of rats with spinal cord injury. METHODS: Thirty Wistar rate were randomly divided into three groups: control group, injury group and NSCs transplantation group, with 10 rats in each group. The models of L4 plane with full transection of spinal cord were made in the rats in injury group and NSCs transplantation group. The cultured NSCs suspension of rats was injected into the injured spinal cord in transplantation group while equal volume saline was injected into the rats in injury group. Two months after operation, the motor and conduction functions of the spinal cord were determined using BBB scores cortical somatosensory evoked potential (CSEP) and horseradish peroxidase (HRP) retrograde tracing. RESULTS: By BBB scores, recovery was observed in the rats in injury group and NSCs transplantation group, but not to the normal level. The recovery in NSCs transplantation group was significantly better than that in injury group. CSEP in both injury group and transplantation group disappeared. The waveform in transplantation group was recovered and the latency tune of CSEP in NSCs transplantation group was prolonged. HRP labeled neurons were found in the anterior horn of the spinal cord in the control group but no neuron was found in the injury group. Some HRP labeled neurons were found in the NSCs transplantation group, but the number of HRP labeled neurons was fewer than that in control group. CONCLUSION: NSCs transplantation improves the partial recovery of motor and conduction functions of injured spinal cord in rats.
基金项目
国家自然科学基金(30572394/C03050302)
重庆市卫生局资助项目(05-2-049)
NETL
NSTL
维普
万方数据