Study of matrine injections on colorectal carcinoma cell line LoVo transplanted subcutaneously in nude mice
AIM: To investigate the effect and the mechanism of matrine injections on the growth of human colorectal carcinoma cell line LoVo transplanted subcutaneously in nude mice. METHODS: Nude mouse model of human colorectal carcinoma by subcutaneous transplantation of LoVo cell line was reproduced. A total of 30 mice were divided into 3 groups: control group, low dosage group and high dosage group. The growth of the subcutaneously transplanted tumor and changes in mouse body weight in each group after treatment were observed on 3, 7, 14 and 21 days. Histopathological examination of xenograft, the heart, the liver, the lungs and the kidneys of nude mice was also conducted. Proliferating cell nuclear antigen and CD34 were detected by im-muhischemmical method. Apoptosis index was detected by terminal dUTP nick end labeling (TUNEL) technique. RESULTS: Matrine injections had significant inhibitory effects on the growth of the transplanted xenograft in vim. Tumor volume, tumor weight, PCNA and microvessel density decreased (P < 0.05) and the apoptosis index increased ( P < 0. 05). The size of necrotic areas was found enlarged significantly in matrine injections - treated group. Matrine injections had no significant effects on the weight of nude mice and nor on the induction of significant histopathological changes in the heart, the liver, the lungs and the kidneys of nude mice. CONCLUSION: Matrine injections have inhibitory effects on the growth of human colorectal carcinoma transplanted subcutaneously in nude mice. The probable mechanism may be related with suppressing angiogenesis and inducing apoptosis of human colorectal carcinoma.
NETL
NSTL
万方数据
维普
