C-Rel与Bcl-xL在脑缺血再灌注大鼠脑组织中的表达及意义
Expression of C-Rel and Bcl-xL in rat brain with focal cerebral ischemia-reperfusion and its significance
龚家明 1罗勇 1王咏龙 1何兰英 1蔡志友1
作者信息
- 1. 重庆医科大学附属第一医院神经内科,重庆市神经病学重点实验室,重庆,400016
- 折叠
摘要
目的:检测脑缺血再灌注大鼠脑组织C-Rel与Bcl-xL表达,探讨NF-кB信号通路在缺血性脑损伤中的作用机制.方法:大脑中动脉线栓法制作脑缺血再灌注动物模型,随机分为正常组(N组)、假手术组(S组)、缺血2 h后再灌注2h组(M2组)、6 h组(M6组)、12 h组(M12组)、24 h组(M24组).RT-PCR和免疫组织化学法检测C-Rel与Bcl-xL,用图像分析仪测定吸光度值.结果:模型组与正常组、假手术组比较,C-Rel与Bcl-xL表达增高(P<0.01).4个模型组比较无统计学差异.C-Rel与Bcl-xL表达有正相关性.结论:C-Rel与Bcl-xL在脑缺血再灌注大鼠脑组织表达增高,NF-кB家族C-Rel通过增强抗凋亡基因Bcl-xL表达发挥脑保护作用.
Abstract
AIM: To investigate the expression of C-Rel and Bcl-xL in Wistar rats with focal cerebral ischemia-reperfusion injury and to study the pathophysiological mechanism of signal pathway of nuclear factor-kappa B in cerebral ischemia injury. METHODS: Wistar rat models of focal cerebral ischemia-reperfusion injury were established by middle cerebral artery occlusion (MCAO) and were randomly divided into normal group (N) , sham-operation group ( S) and cerebral ischemia reperfusion groups(M) , which were subdivided into 2 h reperfusion group (M2 ) , 6h reperfusion group ( M6), 12 h reperfusion group (M12) and 24 h reperfusion group (M24). Expression of C-Rel and Bcl-xL was measured by RT-PCR and immunohistochemistry. The absorbance value was measured by imaging analysis. RESULTS: Expression of C-Rel and Bcl-xL in M groups was higher than that in N and S groups (P <0.01) but no inter-group difference was found in expression of C-Rel and Bcl-xL between the 4 model groups (P > 0. 05). The level of C-Rel had positive correlation with Bcl-xL. CONCLUSION: Expression of C-Rel and Bcl-xL increases in cerebral ischemia-reperfusion injury rats. C-Rel exerts some cerebral protective effects through enhancing the expression of Bcl-xL.
关键词
脑缺血/再灌注/NF-кB/信号传导/细胞凋亡Key words
brain ischemia/reperfusion/NF-kappa B/signal transduction/apoptosis引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据