Anticonvulsant efficacy of Chinese Materia Medica Cynanchum Chinese R. Br in mice
AIM: To study the anticonvulsant efficacy of the water and the chloroform extract from the whole plant of the Cynanchum Chinese R. Br. METHODS: The anticonvulsant activity of the extracts was evaluated by the maximal electroshock ( MES) test and the metrazol seizure test (MET). The minimal neurotox-icity of the extracts was measured by the rotorod test. The independent activity test and the hypnotic synergism test of under threshold hypnotic dosage of pentobarbital were employed to evaluate the central pharmacological effect of the extracts. RESULTS: The water extract exhibited significant protective effect in MET and produced ED50 value of 2.34 g /kg, while the chloroform extract produce no protective effect in the seizure model. On the other hand, the chloroform extract produced protective effects in MES and the ED50 value was 1. 34 g/kg, but the water extract produced no protective effect in the same model. The extracts'exhibited significant dose-dependent inhibitory effects on the spontaneous motor activity in mice after the intraperitoneal administration. Both extracts promoted the hypnotic effect of under threshold hypnotic dosage administration of pentobarbital. The water extract produced ED50 value of 2.36 g /kg and the chloroform extract produced ED50 value of 0.75 g /kg. Compared with phenytoin, the two extracts produced no neurotoxicity in the experimental process. CONCLUSION: Both the water and the chloroform extracts from Cynanchum Chinese R. Br produce some inhibitory effects on CNS, though the two extracts are of different anticonvul- sant properties in different seizure models in mice. Cynanchum Chinese R. Br has a wider application in clinic treatment of epileptic seizures.
cynanchum Chinese R. Branticonvukantsindependent activityhypnotics and sedatives
NETL
NSTL
万方数据
维普
