Biological difference between keratinocytes derived from follicles and epidermis
AIM: To investigate the different characteristics between the keratinocytes derived from follicles and those from epidermis and to discuss their effect in the wound healing process. METHODS: The keratinocytes were harvested respectively from the scalp follicles and the skin epidermis. Having been cultured in K-SFM serum free culture medium in vitro, the keratinocytes were identified by β1-intergrin and keratin15. The third passage was used to explore the characteristics of the growth kinetic. The cell cycle was determined with flow cytometry and the colony forming efficiency (CFE) was studied. RESULTS: The keratinocytes derived from the follicles were positive by β1-intergrin and kera-tin15 staining while the keratinocytes from the epidermis were mostly negative. The cell cycle profile analysis showed that the ratio of S stage in the keratinocytes from the follicles was significantly higher than that in the keratinocytes from the epidermis (23. 44% vs 11.33% , P<0.05). The CFE of the keratinocytes from the follicles was 15.3% and that of the control group was 6.7% (P<0. 05). CONCLUSION: The keratinocytes derived from the follicles have some characteristics of the epidermal stem cells and their proliferative potential is higher compared with that of the keratinocytes from the skin epidermis.
NETL
NSTL
万方数据
维普
