Effect of DDP treatment on ultraweak luminescence in human cervical carcinoma HeLa line
AIM: To study the changes of human cervical carcinoma Hela line in ultraweak luminescence and the proliferation activation following DDP treatment. METHODS: Hela cells were selected and exposed to different concentrations of DDP. The activation of the cell line, the percentage of apoptosis and the changes of cell cycle were detected by MTT and flow cytometry at different time points. The intensity of ultraweak luminescence was examined by IFFM-D chemiluminescence analysis. RESULTS: DDP inhibited the growth of Hela cell line and the effects were time-and concentration-depended. The IC50 of DDP was 3 mg/L at the 48 h point and DDP had no significant cytotoxicity on Hela cell line when its concentration was less than 3 mg/L. The cytotoxicity increased with the enhanced concentration of DDP. After the treatment of DDP, the percentage of apoptosis was (11.4±5.8)%, (21.8 ±7.9)% and (32.5 ±11.6)% respectively at the 24, 48 and 72 h time points. The number of cells increased in G2 phase and decreased remarkably in G1, S phase( P < 0.01). The intensities of ultraweak luminescence also significantly reduced at different time points( P < 0. 01). CONCLUSION: The intensities of ultraweak luminescence in Hela cell line are reduced after the treatment of DDP at non-toxicity dosage, suggesting that the ultra weak luminescence can be used as an indicator in screening sensitive chemotherapeutic agents.
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万方数据
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