Effects of moderate static magnetic field on cell proliferation and cell cycle of leukemia cells
AIM: To investigate the effects of 0.2-0.4 T moderate static magnetic field ( MSMF) on the cell proliferation and cell cycle of human and murine leukemia cells. METHODS: Both Jurkat clone B6-1 and L1210 cells were exposed to moderate static magnetic field(0.2-0.4 T) for 1, 2, 3, 4 and 5 d. Cell morphology was observed by microscopy, proliferation capacity was measured by Cell Counting Kit-8(CCK-8) assay, and cell cycle was detected by flow cytometry ( FCM). RESULTS: After 3 d exposure, the cell cluster of Jurkat clone E6-1 was smaller compared with that in control group, but L1210 cells still grew as single cells. When initial cell density was 4 × 107 cells/L, the proliferation of both cell lines was inhibited significantly after 1, 2 and 3 d exposure, compared with that in control group. Jurkat clone E6-1 cell proliferation was inhibited by 14. 58% , 39. 88% and 6.77% (P<0.05), respectively, and L1210 cell proliferation was inhibited by 8.82% , 10.81% and 5.40% (P <0.05). After 4 and 5 d exposure, the proliferation of both cell lines was enhanced by MSMF. Jurkat clone E6-1 cell proliferation was inhibited by -8.21% (P<0. 05) and -2. 49% (P >0. 05). L1210 cell proliferation was inhibited by -13.01 % and -19.46% (P < 0.05). When initial cell density was 8 × 107 cells/L, the results were in contrast with those of 4 × 107 cells/L. Compared with that in control group, after 1, 2 and 3 d exposure, the proliferation of both cell lines was enhanced by MSMF. The proliferation of Jurkat clone E6-1 cells was inhibited by - 12. 64% , - 20. 36% , -61.43% (P < 0. 05), and the proliferation of L1210 cells was inhibited by - 14. 08% , - 13.09% and - 3. 18% (P < 0. 05). After 4 and 5 d exposure, the proliferation of both cell lines was inhibited. The proliferation of Jurkat clone E6-1 cell was significantly inhibited by 42. 20% and 95. 32% (P < 0. 05 ) , and the proliferation of L1210 cell was inhibited by 46.99% and 41. 97% (P<0.05). After 4 d exposure, the proportion of cells in S phase of Jurkat clone E6-1 increased from 44.94% to 77.92% , while the percent of G2 phase in L1210 cells increased from 5. 28% to 9.22% , compared with those in control group. CONCLUSION: 0.2-0.4 T moderate static magnetic field affects cell proliferation and cell cycle of both Jurkat clone E6-1 and L1210, and the inhibitory effect is related to both cell density and exposure time.
static magnetic fieldleukemiacell proliferationcell cycle
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NSTL
万方数据
维普
