Inhibitory effect of c-erbB2 antisense oligodeoxynucleotide combined with taxol on proliferation of SK-Br-3 cells
AIM : To explore the inhibitory effect on human breast cancer SK-Br-3 cells treated with c-erbB2 antisense oli-godeoxynucleotide(ASODN) combined with taxol. METHODS: SK-Br-3 cells were treated with ASODN, taxol, herceptin, Epiru-bicin, ASODN combined with taxol, herceptin combined with taxol, and Epirubicin combined with taxol for 72 h respectively. The inhibitory effect was detected by MTT assay. The drug's interactional effect was determined by median-effect principle. The expression of c-erbB2 and Bcl-2 was detected by western blot in SK-Br-3 cells. RESULTS: The inhibition ratio of each group increased while the increased concentration of drugs used. The median concentration of taxol was 0.14 μmol/L in taxol group and 0.07 μmol/L in ASODN combined taxol group respectively. The combination index(CI) of ASODN combined with taxol group was always less than 1, indicating that there was some synergistic effects between ASODN and taxol. The expression of c-erbB2 and Bcl-2 proteins was significantly lower in ASODN + taxol group than that in other groups and the difference was statistical significance (P<0.05). CONCLUSION: ASODN significantly enhances the inhibitory effect of taxol on human breast carcinoma SK-Br-3 cells and markedly lowers the taxol's dosage of administration.
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万方数据
维普
