Effect of ketamine, midazolam and propofol on intracellular Ca2+ in developing neurons
AIM: To investigate the effect of ketamine, midazolam and propofol on intracellular Ca2+ in developing primary hippocampus neurons of rats. METHODS: On the fifth day, the cultured hippocampal neurons were co-incubated with 10 mμol/L Fluo-4 AM for 30 min at 37 ℃. Excess dye was removed with three rinses of DMEM. Fluorescence imaging of intracellular Ca2+ was performed to detect a number of neurons selected when the hippocampal neurons were exposed to 150 μmol/L ketamine, 3 μmol/L midazolam or 10 μmol/L propofol respectively. RESULTS: After exposed to 150 μmol/L ketamine, the intracellular calcium concentration of the developing neurons decreased significantly and fluorescence intensity of the neurons decreased from(987±307) to (766 ±226)(P<0.05). When exposed to 3 μmol/L midazolam or 10 μmol/L propofol, the cytosolic Ca2+ of the neurons increased significantly and fluorescence intensity of the neurons exposed to midazolam increased from( 1707 ±514) to (2663 ± 572) (P < 0.05). Fluorescence intensity of the neurons exposed to propofol increased from( 1057 ± 353) to ( 1749 ± 708 ) (P<0.05). CONCLUSION: Ketamine decreases the intracellular Ca2+ of rat developing primary hippocampus neurons while midazolam and propofol may increase the intracellular Ca2+ of the neurons.
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万方数据
维普
