氯胺酮、咪达唑仑及丙泊酚对发育期神经元细胞内钙的影响
Effect of ketamine, midazolam and propofol on intracellular Ca2+ in developing neurons
谭蕾 1罗爱林 1向强 1赵以林 1何璇1
作者信息
- 1. 华中科技大学同济医学院附属同济医院麻醉学教研室,湖北,武汉,430030
- 折叠
摘要
目的:探讨氯胺酮、咪达唑仑及丙泊酚对大鼠发育期海马神经元细胞内钙浓度的影响.方法:将原代培养第5 日的大鼠海马神经元用10umol/L的Ca2+指示剂Fluo-4共孵育30 min洗涤后,分别加入150 umol/L氯胺酮,咪达唑仑3umol/L,丙泊酚10 umol/L,采用激光共聚焦显微镜选定多个细胞分别测定荧光强度的变化.结果:氯胺酮使体外培养第5日的海马神经元代表钙浓度的荧光强度明显下降[(987±307)vs(766±226),P<0.05],咪达唑仑,丙泊酚均使体外培养5 d的海马神经元荧光强度明显升高[(1707±514)vs(2663±572),(1057 ±353)vs(1749 ±708),P<0.05].结论:阻滞NMDA受体的氯胺酮降低发育期海马神经元细胞内钙浓度,而兴奋GABAA受体的咪达唑仑,丙泊酚则会升高细胞内钙浓度.
Abstract
AIM: To investigate the effect of ketamine, midazolam and propofol on intracellular Ca2+ in developing primary hippocampus neurons of rats. METHODS: On the fifth day, the cultured hippocampal neurons were co-incubated with 10 mμol/L Fluo-4 AM for 30 min at 37 ℃. Excess dye was removed with three rinses of DMEM. Fluorescence imaging of intracellular Ca2+ was performed to detect a number of neurons selected when the hippocampal neurons were exposed to 150 μmol/L ketamine, 3 μmol/L midazolam or 10 μmol/L propofol respectively. RESULTS: After exposed to 150 μmol/L ketamine, the intracellular calcium concentration of the developing neurons decreased significantly and fluorescence intensity of the neurons decreased from(987±307) to (766 ±226)(P<0.05). When exposed to 3 μmol/L midazolam or 10 μmol/L propofol, the cytosolic Ca2+ of the neurons increased significantly and fluorescence intensity of the neurons exposed to midazolam increased from( 1707 ±514) to (2663 ± 572) (P < 0.05). Fluorescence intensity of the neurons exposed to propofol increased from( 1057 ± 353) to ( 1749 ± 708 ) (P<0.05). CONCLUSION: Ketamine decreases the intracellular Ca2+ of rat developing primary hippocampus neurons while midazolam and propofol may increase the intracellular Ca2+ of the neurons.
关键词
氯胺酮/咪达唑仑/丙泊酚/原代培养/海马神经元/钙离子浓度Key words
ketamine/midazolam/propofol/primary culture/hippocampus neuron/calcium concentration引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据