Changes in gap junction-mediated intercellular communication of rabbit lens epithelia cells irradiated by UVA
AIM: To probe the changes in gap junction-mediated intercellular communication ( GJIC ) of rabbit lens epithelia cells(RLECs) irradiated by UVA. METHODS: 2-3 generation RLECs were placed in 12-well tissue culture plates and incubated for 24 hours. These cells were divided into normal control group and ultraviolet irradiation group. The UV intensity was 0. 2 mW/ cm2 under a UV lamp with fixed position-50 cm and RLECs were exposed to ultraviolet lamp for different times: 5 minutes, 10 minutes and 15 minutes. Fluorescence redistribution after photob-leaching ( FRAP) was used to evaluate the functions of GJIC through a laser canning confocal microscope. RESULTS: The GJIC function of normal control group and ultraviolet irradiation group was examined by FRAP. The mean fluorescence recovery rate of the cells in control group was(58. 337 ±5. 620)%. We found that the mean fluorescence recovery rate was gradually declining with the increase of irradiation time [ ( 34. 205 ± 3.652)%, 18.809 ±3.017)% and (7.029 ±2.917)% respectively]. Compared with that in control group, the mean fluorescence recovery rate of ultraviolet irradiation group was statistically different (P<0.01). CONCLUSION: UVA irradiation reduces gap junction-mediated intercellular communication of rabbit lens epithelia cells and the function decreases with lengthening time of radiation.
lens epithelia cellultraviolet raysfluorescence redistribution after photobleachinggap junction-mediated intercellular communication
NETL
NSTL
万方数据
维普
