摘要
目的:观察近紫外线对兔晶状体上皮细胞通讯功能的影响.方法:对兔晶状体前囊膜行组织块细胞培养,将传代2～3代兔晶状体上皮细胞在12孔板中培养24 h后,分为正常对照组和近紫外线照射组(同一近紫外线光源下一固定位置50 cm,测得该点的功率为0.2 mW/cm2,给于同一强度,5,10,15 min不同时间的近紫外线照射,采用荧光光漂白恢复技术(FRAP),通过激光共聚焦显微镜检测各组兔晶状体上皮细胞的缝隙连接细胞间通讯(GJIC)功能变化情况.结果:FRAP测定正常对照组和近紫外线照射组细胞GJIC功能,正常对照组细胞平均荧光恢复率为(58.337±5.620)%,随着照射时间的延长,近紫外线照射组细胞平均荧光恢复率逐渐下降,分别为(34.205±3.652)%,(18.809 ±3.017)%,(7.029±2.917)%,与正常对照组相比较差异具有统计学意义(P<0.01).结论:近紫外线照射能降低兔晶状体L皮细胞GJIc功能,随着照射时间的增加,其功能下降越明显.
Abstract
AIM: To probe the changes in gap junction-mediated intercellular communication ( GJIC ) of rabbit lens epithelia cells(RLECs) irradiated by UVA. METHODS: 2-3 generation RLECs were placed in 12-well tissue culture plates and incubated for 24 hours. These cells were divided into normal control group and ultraviolet irradiation group. The UV intensity was 0. 2 mW/ cm2 under a UV lamp with fixed position-50 cm and RLECs were exposed to ultraviolet lamp for different times: 5 minutes, 10 minutes and 15 minutes. Fluorescence redistribution after photob-leaching ( FRAP) was used to evaluate the functions of GJIC through a laser canning confocal microscope. RESULTS: The GJIC function of normal control group and ultraviolet irradiation group was examined by FRAP. The mean fluorescence recovery rate of the cells in control group was(58. 337 ±5. 620)%. We found that the mean fluorescence recovery rate was gradually declining with the increase of irradiation time [ ( 34. 205 ± 3.652)%, 18.809 ±3.017)% and (7.029 ±2.917)% respectively]. Compared with that in control group, the mean fluorescence recovery rate of ultraviolet irradiation group was statistically different (P<0.01). CONCLUSION: UVA irradiation reduces gap junction-mediated intercellular communication of rabbit lens epithelia cells and the function decreases with lengthening time of radiation.
NETL
NSTL
维普
万方数据