顺铂作用于宫颈癌HeLa细胞致细胞周期调节因子变化及对细胞周期的阻滞作用
Changes of cell cycle regulators and cell cycle arrest in DDP-treated HeLa cells
朱克修 1李玢 1于翠革 2曹亚莉 1王佳 1韩晓兵1
作者信息
- 1. 西安交通大学医学院第一附属医院妇产科,陕西,西安,710061
- 2. 陕西省人民医院妇产科,陕西,西安,710068
- 折叠
摘要
目的:探讨细胞周期调节因子atm,chk2,p53基因在顺铂(DDP)诱导宫颈癌HeLa细胞凋亡中的作用.方法:MTT法检测梯度浓度DDP对HeLa细胞的生长抑制率,RT-PCR法和Western Blot法检测DDP作用前后HeLa细胞atm,chk2及p53 mRNA和蛋白的表达.流式细胞仪检测细胞凋亡及细胞周期的变化.结果:DDP对宫颈癌HeLa细胞的生长抑制作用具有浓度和时间依赖性.DDP作用HeLa细胞后atm,chk2,p53 mRNA和蛋白的表达均增强,细胞凋亡增加,细胞周期阻滞于G2/M期(P<0.05).结论:atm,chk2,p53基因与细胞凋亡及G2/M期阻滞有关.干预atm,chk2,p53基因通路有望成为宫颈癌化疗增敏的新策略.
Abstract
AIM: To investigate the possible roles of the cell cycle regulators atm, chk2 and p53 in DDP induced-apoptosis in HeLa cells. METHODS: The proliferation inhibitory rates of HeLa cells induced by different concentrations of DDP were observed by MTT assays. The mRNA and protein expressions of atm, chk2 and p53 in HeLa cells treated with or without DDP were detected by RT-PCR and Western blot, respectively. Cell cycle analysis was performed by flow cytometry. RESULTS: DDP inhibited the proliferation of HeLa cells in a dose and time dependent manner. The mRNA and protein expressions of atm, chk2 and p53 increased in DDP-treated HeLa cells. The apoptosis increased and the cell cycle was arrested in G2/M phase in DDP-treated HeLa cells (P < 0. 05). CONCLUSION: The activation of atm, chk2 and p53 may be related to apoptosis and cell cycle arrest. Targeting ATM, chk2 and p53 pathway may be a promising strategy to increase the sensibility to DDP in cervical cancer.
关键词
atm基因/chk2基因/p53基因/细胞周期/宫颈肿瘤/顺铂Key words
atm gene/chk2 gene/p53 gene/cell cycle/cervical neoplasms/DDP引用本文复制引用
基金项目
陕西省科技计划项目(2006K09-G11-3)
出版年
2009
NETL
NSTL
维普
万方数据