Influence of interactions between lung cancer cells and fetal lung fibroblast on matrix metalloproteinase-2 expression
AIM To investigate the influence of interactions between lung cancer cell H460 and fetal lung fibroblasts on matrix metalloproteinase-2 ( MMP-2 ) expression. METHODS: H460 cells with condition medium derived from fetal lung fibroblasts or fetal lung fibroblasts with condition medium derived from H460 cells were incubated in monolayer culture model. Fetal lung fibroblasts and H460 cells at 1:1, 1 : 2, 1:4 and 1:8 cell ratios were co-cultured in monolayer culture model. H460 cells with fetal lung fibroblasts were co-cultured in three dimensional collagen gels. After 72 hours, the activity and expression of MMP-2 in the condition medium were studied by gelatin zymography. RESULTS: The condition medium derived from H460 cells stimulated proMMP-2 and MMP-2 expression while the condition medium derived from fetal lung fibroblasts failed to stimulate proMMP-2 and MMP-2 of H460 cells. When fetal lung fibroblast and H460 cells were co-cultured at 1:1, 1:2 and 1:8 cell ratios, the levels of both proMMP-2 and MMP-2 were enhanced and at 1:4 only the levels of pro-MMP-2 were enhanced. H460 cells cultured with fetal lung fibroblasts resulted in the production of proMMP-2 and MMP-2 and the production in three dimensional collagen gels was higher than that in monolayer culture model. CONCLUSION: The interactions between lung cancer cell H460 and fetal lung fibroblasts may influence lung cancer invasion and metastasis through up-regulating MMP-2 secretion and activation.
lung cancer cellfetal lung fibroblasttissu culture techniquematrix metalloproteinase-2metastasis
NETL
NSTL
万方数据
维普
