Effect of substance P on expression of Osterix in osteoblast differentiation
AIM: To explore the molecular pathway of substance P-induced osteoblast differentiation. METHODS: Mesenchymal stem cells of rats(rMSCs) were isolated, cultured and induced to differentiate osteoblast. Under the interference of substance P or/ and substance P antagonist L732138, total RNA was extracted and Osterix gene expression was evaluated by RT-PCR. RESULTS: Compared with that in normal control group, the expression of Osterix increased significantly in substance P group(LSD-t =0.117, P <0.01) in the course of osteoblast differentiation, but no significant difference was seen compared with that in substance P receptor antagonist L732138 group ( LSD-t = 0. 008, P > 0. 05 ). Compared with that in substance P group, the Osterix expression decreased significantly in the federal group of substance P and substance P receptor antagonist L732138 (LSD-t = -0. 172, P < 0.01). CONCLUSION: Substance P stimulates the expression of Osterix, which can be depressed by substance P receptor antagonist L732138. Substance P stimulates osteoblast differentiation by stimulating the expression of Osterix.
NETL
NSTL
万方数据
维普
