Effect of zinc on expression of EGF and EGFR in scalded rats
AIM: To observe the effect of zinc on expression of EGF and EGFR in zinc-deficient scalded rats. METHODS: Sixty-four SD rats were inflicted by 20% total body surface area (TBS A) with deep partial thickness burn and then were randomly divided into simple scald group and zinc treatment group. On 1, 3, 5 and 7 days after scald, the contents of serum zinc were measured, biopsies were harvested for the pathomrphological examination and the expression of EGF and EGFR were detected by SABC immunohistochemical method. The above indicators were also employed in another 8 normal rats used as normal control group. RESULTS: On the first day after scald, the serum zinc of simple scald group reduced (0.097 ±0.006) mg/L, lower than that in normal control group[ (0. 133 ±0. 005) mg/L, P <0. 01 ], and returned to normal 7 days later. On the first day after scald, the serum zinc of zinc treatment group was not significantly different from that in control group (P > 0.05 ) , but on the 3 rd, 5 th and 7 th day, the contents of serum zinc were (0. 148 ± 0. 007), (0.164 ± 0. 006 ) and (0. 172 ± 0. 007 ) mg/L, significantly increased and higher than those in normal control group and those in simple scald group (P < 0. 01 ). EGF and EGFR were not or only weakly expressed in the normal skins and wound tissues on the first day after scald. In both simple scald group and zinc treatment group, the expression of EGF increased on the 5 th and the 7 th days after scald, but the expression in zinc treatment group was higher than that in simple scald group (P < 0. 05 ). In both simple scald group and zinc treatment group, the expression of EGFR increased on the 3 rd, the 5 th and the 7 th day after scald. However the expression of EGFR in zinc treatment group was higher than that in simple scald group ( P < 0. 05 ). CONCLUSION: Zinc promotes the expression of EGF and EGFR in scalded rats and accelerates the wound healing.
NETL
NSTL
万方数据
维普
