Effect of different dosed estrogen on expression of intercellular adhesion molecule-1 and tumor necrosis factor-after focal cerebral ischemia/reperfusion in rats
AIM: To study the neuroprotective role of different dosed estrogen on expressions of ICAM-1 and TNF-α after focal cerebral ischemia/reperfusion in male rats. METHODS: Totally 200 male SD rats, with the body mass of 250-300 g, were selected and randomly assigned into 5 groups: Sham operation group(SO), ischemia reperfusion group( I/R), low-dosage estrogen treatment group ( EA), medium-dosage estrogen treatment group (EB) and high-dosage estrogen treatment group (EC). The expression of ICAM-1 and TNF-α was detected by immunohisto-chemistry. Data in all groups were analyzed with test. RESULTS: The data of the 200 rats were analyzed. The expression of ICAM-1 and TNF-α in the I/R group(94.62 ±5.72) and (41.68 ±2.46) were higher than those in the SO group(2. 32 ± 0.41 and 1. 88 ± 0. 28) and treatment groups (68. 28 ± 4. 14), (39.35 ± 6. 63), (40. 90 ± 6. 09) and (19. 22 ± 0. 96), (11.92 ± 1. 36),(12. 75 ± 1.03), respectively. The estrogen level in EA group was significantly higher than those in the EB group and EC group (P < 0. 05 ) , but there was no significant difference between the EB group and the EC group ( P > 0. 05). The ICAM-1 expression was observed on cerebral cortex in I/R group and different treatment groups after 2-hour cerebral ischemia and 3-hour reperfusion. The expression increased significantly after 12 th and reached the peak at 24 th. CONCLUSION: Estrogen may exert some neuroprotective effect against the cere- bral ischemia in rats by inhibiting the activation of TNF-α and decreasing the expression of ICAM-1. The neuroprotective effect of estrogen is closed related to its dosage.
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