目的:观察小鼠心、肝、脾、肺、肾等器官干扰素诱导蛋白IFIT1(Interferon-induced protein with tetratricopetide repeats 1)的表达情况,探讨颈交感神经阻滞(cervical sympathetic block,SB)对烧伤后早期小鼠各器官iFIT1表达状况的影响.方法:将雄性昆明小鼠18只随机分为正常对照组、烧伤组和颈交感神经阻滞(SB)治疗组、烧伤组和SB治疗组采用体表总面积(Total body surface area,TBSA)15%～20%Ⅲ度小鼠烧伤模型,SB治疗组于烧伤后即刻进行双侧颈交感神经阻滞,各组分别于4h后取心、肝、脾、肺、肾组织,提取RNA,以RT-PCR检测WIT1表达情况.结果:RT-PCR显示IFIT1在各组的各器官均有表达,并存在差异.烧伤组IFIT1在心、肝、脾表达低于正常组,同时在肺、肾表达高于正常组,差异显著(P＜0.01).SB治疗组IFIT1的表达均低于正常组(P＜0.01);同时,SB治疗组IFIT1在心、肝、肺、肾的表达也低于烧伤组(P＜0.01).另外,在各组内的不同器官的IFIT1表达量存在差异(P＜0.05).结论:烧伤后早期小鼠各器官IFIT1表达有不同的变化,可能与不同器官在烧伤应激反应过程中损伤程度不同有关.SB可显著降低各器官IFIT1的表达.
Effect of cervical sympathetic block on Interferon-induced protein IFIT1 levels in organs of burned mice during early stage
AIM : To observe the expression of IFTT1 (Interferon-induced protein with tetratricopetide repeats 1) in the heart, liver, spleen, lungs, kidneys of mice and to detect the effect of cervical sympathetic block (SB) on IFTT1 of organs during the early stage in burned mice. METHOD: Eighteen male Kunming mice were divided randomly into control group, burned group and SB group. Burned group and SB group were inflicted with 15% -20% TBSA (Total body surface area) full thickness bum injury and SB group was treated immediately by SB. Tissues of the heart, liver, spleen, lungs and kidneys were isolated 4 h post-burn. The RNA expression level of IFTT1 was measured by RT-PCR. RESULTS: The RNA expression levels of IFTT1 in burned group decreased significantly in the heart, liver and spleen and significantly increased in the lungs and kidneys compared with those in control group (P <0. 01). The RNA expression levels of IFTT1 in SB group decreased significantly in all the organs examined compared with those in control group( P < 0.01) and decreased significantly in the heart, liver, lungs and kidneys compared with those in burned group(P < 0.01). Difference was also found in the RNA expression levels of IFTT1 between the differenct organs examined in each group( P < 0.05 ). CONCLUSION: In organs of burned mice during early stage, the changes of RNA expression level of IFTT1 vary with different organs, which may be related to the different degrees of organ injury during stress reaction process. SB can significantly decrease the expression of IFTT1 in organs involved.
NETL
NSTL
万方数据
维普
