Effects of Genistein on morphological changes in cultured hippocampal neurons following β-Amyloid(25-35) induced apoptosis
AIM: To investigate the neuroprotective effect of Genistein on morphological changes in cultured hippocampal neu-rons following β-Amyloid(25-35)-induced apoptosis. METHODS: The model of damage was induced by Aβ25-35 and the changes of cell morphology were observed. Apoptosis in hippocampal neurons induced by Aβ25-35 was detected with hoechst33342 and PI co-staining method. NSE and Tunel double-staining assay were also conducted. RESULTS: Compared with that in Aβ25-35 only group, the morphology damage of hippocampal neurons in Genistein model group was less severe and apoptosis in Genistein model group was obviously lower. CONCLUSION: Genistein, with some neuro-protective or anti-apoptotic effects, plays an important role in up-regulating the hippocampal neuronal survival during the process of Aβ25-35-induced damage.
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