bFGF对局部性脑缺血再灌注大鼠海马及皮质神经元CREB表达的影响
Effects of bFGF on expression of CREB in hippocampus and cortex during cerebral ischemia and reperfusion in rats
曾朝涛 1郭和 1邢雪松 1吕威力2
作者信息
- 1. 沈阳医学院基础医学院解剖教研室,辽宁,沈阳,110034
- 2. 沈阳医学院基础医学院病理教研室,辽宁,沈阳,110034
- 折叠
摘要
目的:研究大鼠脑缺血再灌注后cAMP反应元件结合蛋白(CREB)表达的变化,探讨碱性成纤维细胞生长因子(bFGF)对脑组织缺血再灌注神经元CREB的调节作用及机制.方法:应用线栓法制作大鼠局灶性脑缺血再灌注模型,大脑中动脉阻塞2 h再灌注损伤24 h,采用TUNEL法、免疫组化检测海马及皮质内神经元凋亡和CREB的表达.结果:大鼠缺血再灌注海马及顶叶皮质内神经元凋亡数增加而CREB表达较假手术组减少,注射bFGF后神经元凋亡数减少.CREB表达明显高于缺血再灌注组.结论:bFGF抑制缺血神经元凋亡.参与CREB的调节,对缺血神经元有保护作用.
Abstract
AIM: To investigate the expression of cyclic AMP response element binding protein ( CREB) in hippocampus and cortex after cerebral ischemia and reperfusion in rats and to explore the regulative effects of basic fibroblast growth factor ( bF-GF)on CREB in brain tissue and its mechanism. METHODS: The rat models of middle cerebral arteries occlusion ( MCAO) were established with intraluminal filament blockade. The expression of CREB and apoptosis of neurons in hippocampus and cortex were detected with immunohistochemistry and TUNEL method respectively. RESULTS: The expression of CREB in hippocampus and cortex decreased after cerebral ischemia and reperfusion. In bFGF group, the expression of CREB increased while cell apoptosis decreased. CONCLUSION: bFGF depresses cell apoptosis, participates in the regulation of CREB expression in ischemic neurons and protects ischemic neurons.
关键词
脑缺血/成纤维细胞生长因子2/CREB/海马/细胞凋亡Key words
brain ischemia/fibroblast growth factor 2/CREB/hippocampus/apoptosis引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据