摘要
目的:观察CD44在小鼠磨牙牙胚发育表达的时空顺序及分布,探讨CD44对牙齿发育的形态发生和细胞分化的作用及意义.方法:采用免疫组织化学方法,观察CD44在牙胚发育的蕾状期、帽状期、钟状形态发生期、分化期、分泌期、成熟期的表达.结果:在牙胚发育的蕾状期、帽状期,CD44在口腔上皮、牙板上皮、成釉器、牙乳头、牙囊呈弱阳性表达或无表达.在钟状期CD44在口腔上皮、牙板上皮强阳性表达;外釉上皮、星网状层、成牙本质细胞弱阳性表达;分泌期的成釉细胞和牙囊阳性表达.钟状分化期、分泌期、成熟期,中间层细胞CD44强阳性表达.结论:CD44参与牙齿形态发生及细胞分化,推测其在釉质矿化以及牙根和牙周组织的形成中起重要作用.
Abstract
AM:To study the expression and function of CD44 in different development stages of mouse tooth germ. METHODS: Immunohistochemistry was adopted to detect the expression of CD44 at the bud stag, cap stag, bell of initial morphogenesis stage,bell of differetiational stage, bell of secretory stage, and bell of maturative stage of mouse tooth germ. RESULTS: At the bud, cap stages, a weak or negative expression of CD44 was seen in oral epithelial, dental lamina, enamel organ, dental papilla, and dental follicle. During the bell stage, the expression of CD44 was strong positive in oral epithelim, dental lamina, was weak positive in the outer enamel epithelium, stellate reticulum and od-ontoblasts and was positive in the secretory ameloblasts and dental follicle. The expression of CD44 was strong positive in stratum intermedium at the bell of differetiational stage, bell of secretory stage, and bell of maturative stage of mouse tooth germ. CONCLUSION: CD44 is involed in tooth morphogensis and cell differentiation. The results suggest that CD44 may play a significant role in enamel mineralization and be associated with the formation of root and periodontal tissues.
NETL
NSTL
维普
万方数据