Association of G679S polymorphism of glucocorticoid receptor gene and steroid-resistance in asthma
AIM: To investigate the association of glucocorticoid receptor (GR) gene G679S polymorphisms and steroid-resistant asthma. METHODS : G679S polymorphism of GR gene was gen-otyped in 64 steroid-resistant (SR) asthma patients, 217 steroid-sensitive (SS) asthma patients and 221 healthy control subjects by the PCR sequence specific primer (PCR-SSP) technique. The plasma cortisol levels and GR dissociation constant (Kd) were also detected in all the three groups. RESULTS: It was found that the frequencies of GG, GA and AA genotypes of G679S in SR were 46.9%, 42.2% and 10.9% , respectively. They were statistically different in the distributions of the genotypes in SS, 63. 6%, 31.3% and 5. 1% and in healthy control subjects, 65. 6%, 29.0% and 5.4%, respectively (P=0.035, P=0.02). It was also found that GA + AA genotypes and A allele frequencies in SR were significantly higher than those in SS and healthy control subjects. In SR, GR Kd was significantly higher in GA + AA genotypes than that in GG genotype (P =0.029). However, there was no significant difference in the plasma cortisol levels in all the three genotypes. CONCLUSION: The G679S gene polymorphism of GR is associated with steroid-resistant asthma and the allele A may be a risk factor for steroid-resistance in Chinese population.
NETL
NSTL
万方数据
维普
